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| Status |
Public on May 12, 2022 |
| Title |
Adipose tissue poly RNA profiles of C57BL/6N-ΔGm15551 mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cold and nutrient activated brown adipose tissue (BAT) is capable of increasing systemic energy expenditure via uncoupled respiration and secretion of endocrine factors thereby protecting mice against diet-induced obesity and improving insulin response and glucose tolerance in men. Long non-coding RNAs (lncRNAs) have recently been identified as fine tuning regulators of cellular function. While certain lncRNAs have been functionally characterised in adipose tissue, their overall contribution in the activation of BAT remains elusive. We identified lncRNAs correlating to inter- scapular brown adipose tissue (iBAT) function in high fat diet (HFD) and cold stressed mice. We focused on Gm15551 which has an adipose tissue specific expression profile, is highly upregulated during adipogenesis and downregulated by β-adrenergic activation in mature adipocytes. Albeit we performed comprehensive transcriptional and adipocyte physiology profiling in vitro and in vivo, we could not detect an effect of gain or loss of function of Gm15551.
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| Overall design |
PolyA RNA profiles of different adipose tissues from 12-week old ∆Gm15551 and wild type litter mate mice.
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| Contributor(s) |
Engelhard CA, Huang C, Khani S, Kasparek P, Procházka J, Rozman J, Reguera DP, Sedláček R, Kornfeld J |
| Citation(s) |
35645339 |
| Submission date |
Apr 12, 2022 |
| Last update date |
Aug 11, 2022 |
| Contact name |
Jan Wilhelm Kornfeld |
| E-mail(s) |
janwilhelmkornfeld@bmb.sdu.dk
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| Organization name |
University of Southern Denmark
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| Department |
Biochemistry and Molecular Biology
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| Lab |
Noncoding Kornfeld Lab - Principles of Energy Homeostasis
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| Street address |
Campusbej 50
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| City |
Odense |
| ZIP/Postal code |
5230 |
| Country |
Denmark |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE200656 |
Comprehensive transcriptional profiling and mouse phenotyping reveals dispensable role for adipose tissue selective long noncoding RNA Gm15551. |
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| Relations |
| BioProject |
PRJNA825726 |
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