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| Status |
Public on Apr 03, 2023 |
| Title |
Effect of endothelial specific inhibition of Brg1 on gene expression during zebrafish heart regeneration [RNA-seq] |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To decipher the molecular action of endothelial Brg1 in the regulation of zebrafish heart regeneration, we applied Tg(kdrl:eGFP) to label cardiac endothelial cells including the endocardium, and achieved endothelium-specific over-expression of DN-xBrg1 by using the compound zebrafish line consisting of Tg(ubi:loxp-DsRed-STOP-loxp-DN-xBrg1; kdrl:CreER; kdrl:eGFP) (defined as DNK), while we used Tg(ubi:loxp-DsRed-STOP-loxp-DN-xBrg1; kdrl-eGFP) as control (CtrlK) in the presence of 4-HT starting at 3 days before ventricular resection. The kdrl:eGFP endothelial cells, which were sorted by FACS (fluorescence-activated cell sorting) from CtrlK and DNK hearts at 7 dpa, were subjected to RNA-seq analysis.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for CtrlK group and DNK group. There were two biological replicates for each group.
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| Contributor(s) |
Ding W, Hou J, Xiao C, Xiong J |
| Citation(s) |
37029137 |
| Submission date |
Apr 17, 2022 |
| Last update date |
May 03, 2023 |
| Contact name |
Jing-Wei Xiong |
| E-mail(s) |
jingwei_xiong@pku.edu.cn
|
| Organization name |
Institute of Molecular Medicine, Peking University
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| Street address |
Peking University, No.5 Yiheyuan Road Haidian District
|
| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
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| Samples (4)
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| GSM6046883 |
Zebrafish heart endothelial cells, 7dpa, CtrlK1 |
| GSM6046884 |
Zebrafish heart endothelial cells, 7dpa, CtrlK2 |
| GSM6046885 |
Zebrafish heart endothelial cells, 7dpa, DNK1 |
| GSM6046886 |
Zebrafish heart endothelial cells, 7dpa, DNK2 |
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| This SubSeries is part of SuperSeries: |
| GSE200938 |
Effect of endothelial specific inhibition of Brg1 |
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| Relations |
| BioProject |
PRJNA827593 |
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