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| Status |
Public on Dec 12, 2023 |
| Title |
Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for RNA-polymerases encoded by P23-45 bacteriophage |
| Organism |
Thermus thermophilus HB8 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
By doing ChIP-seq with antibodies against two P23-45 RNA polymerases gp64 and gp96 we showed that both RNA polymerases interact with the phage genome at the early stage of infection. Gp96 interacts with pre-early P23-45 genes, while gp64 interacts with pre-early, early and some middle-stage genes.
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| Overall design |
Thermus thermophilus HB8 cells were infected with P23-45 bacteriophage at a high multiplicity of infection. Next, cells were collected at 5, 20, and 40 minutes post infection with the phage. Chromatin immunoprecipitation followed by DNA-sequencing (ChIP-seq) was performed using antibodies against P23-45 RNA polymerases gp64 and gp96 (first 1540 amino acid residues). Samples without addition of antibodies were used as controls for input DNA.
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| Contributor(s) |
Goldobina E, Chaban A, Sokolova M |
| Citation(s) |
38182597 |
| Submission date |
Oct 14, 2022 |
| Last update date |
Jan 17, 2024 |
| Contact name |
Rob Lavigne |
| E-mail(s) |
rob.lavigne@kuleuven.be
|
| Organization name |
KU Leuven
|
| Department |
Biosystems
|
| Lab |
Laboratory of Gene Technology
|
| Street address |
Kasteelpark Arenberg 21
|
| City |
Leuven |
| ZIP/Postal code |
3001 |
| Country |
Belgium |
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| Platforms (1) |
| GPL32745 |
Illumina HiSeq 4000 (Thermus thermophilus HB8) |
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| Samples (21)
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| This SubSeries is part of SuperSeries: |
| GSE215812 |
T. thermophilus infected with phage P23-45 |
|
| Relations |
| BioProject |
PRJNA890650 |
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