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| Status |
Public on Jan 31, 2024 |
| Title |
A new adipocyte-specific long non-coding RNA engages impaired fatty acid sensing and dyslipidaemia in obese subjects (microarray) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The relevance of adipose-derived long noncoding RNAs (lncRNAs) to the regulation of gene expression patterns related to the obese phenotype remains elusive. Here, the analysis of transcriptomes of adipose tissue before-after significant weight loss led to the identification of 496 putative lncRNAs (FDR p-values <0.05). Only the nonconserved linc-GALNTL6-4 displayed a fold-change above 2 plus adjusted p-value <0.0001. Exploration of additional omental and subcutaneous fat samples confirmed diminished linc-GALNTL6-4 in obese subjects, while lncRNA-mRNA co-expression analyses highlighted its association with fatty acid metabolism. Concurrently, the expression of this unique lncRNA towered in ex vivo isolated and in vitro differentiated adipocytes, being compromised under conditions that mimicked inflammation in obese adipocytes. On the other hand, the knockdown of linc-GALNTL6-4 in fat cell progenitors conveys impaired differentiation, fostering deranged expression patterns in differentiated adipocytes, including the increased expression of genes devoted to the transport of lipids, such as Apolipoprotein C1 (APOC1) and the intracellular fatty acid-binding protein FABP3. Conversely, overexpression of linc-GALNTL6-4 ameliorated adipocyte performance through the specific control of the short-chain fatty acid receptor FFAR3. Current data unveil the unforeseen relevance of linc-GALNTL6-4 as a modulator of fatty acid sensing in adipocytes deeply challenged by body weight and meta-inflammation.
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| Overall design |
The increased expression of linc-GALNTL6-4 in primary in vitro differentiated human adipocytes was accomplished by means of a customized plasmid. To this end, the linc-GALNTL6-4 was first obtained and amplified from human liver cDNA, using specific primers with restriction sites for KpnI and FseI (Fwd, 5’-GGTAC* CCTTTTGCAGAAGTAGATGTGCG-3’; Rev, 5’-GGCCGG* CCATTCAAGTGGTTGTTTTATTGACA-3’). The resulting amplicon was purified and cloned into a modified pCMV6 vector (Origene, PS100001). Then, differentiated fat cells were transfected 1:3 with either our construct or an empty plasmid control using the FuGENE® HD Transfection Reagent (Promega).
10 samples were analysed (Control=5, LINC_OE=5)
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| Contributor(s) |
Ortega FJ, Lluch A |
| Citation missing |
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| Submission date |
Nov 28, 2022 |
| Last update date |
Jan 31, 2024 |
| Contact name |
Júlia Perera-Bel |
| E-mail(s) |
mardata-bu@researchmar.net
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| Organization name |
Hospital del Mar Research Institute
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| Department |
MARData-BU
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| Street address |
Doctor Aiguader, 88
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| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
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| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE218897 |
Functional consequences of impaired linc-GALNTL6-4 in adipocytes |
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| Relations |
| BioProject |
PRJNA906095 |
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