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| Status |
Public on Jan 14, 2023 |
| Title |
Next Generation Sequencing Facilitates Transcriptomes Quantitative Analysis of N2A cells transfected with the plasmids respectively encoding RfxCas13d/dRfxCas13d and crRNA. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To determine what effect the collateral activity of RfxCas13d has on N2acells. The plasmids encoding RfxCas13d/dRfxCas13d and crRNA were transfected into N2a cells. After 48 hours of transfection, GFP-positive cells were collected using FCAS and extracted for total RNA. Then, we analyzed the transcriptome of each group with RNA-seq and analyzed the differential genes among the groups.
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| Overall design |
mRNA profiles of N2A cells transfected with the plasmids respectively encoding RfxCas13d/dRfxCas13d and crRNA.
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| Contributor(s) |
Li Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 09, 2023 |
| Last update date |
Jan 14, 2023 |
| Contact name |
Yunfei Li |
| E-mail(s) |
1711110048@bjmu.edu.cn
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| Organization name |
Peking University
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| Department |
Institute of Systems Biomedicine, Department of Immunology, School of Basic Medical Sciences, Beijing Key Laboratory of Tumor Systems Biology, Peking University Health Science Center, Beijing, China
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| Street address |
38 Xueyuan Road, Haidian District, Beijing
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| City |
Beijing |
| State/province |
China |
| ZIP/Postal code |
100191 |
| Country |
China |
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| Platforms (1) |
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| Samples (36)
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| GSM6923679 |
dRfxCas13d_K8 crRNA, rep 1 |
| GSM6923680 |
dRfxCas13d_M1 crRNA, rep 1 |
| GSM6923681 |
dRfxCas13d_M3 crRNA, rep 1 |
| GSM6923682 |
dRfxCas13d_T4 crRNA, rep 1 |
| GSM6923683 |
dRfxCas13d_T6 crRNA, rep 1 |
| GSM6923684 |
dRfxCas13d_N5 crRNA, rep 1 |
| GSM6923685 |
RfxCas13d, rep 1 |
| GSM6923686 |
RfxCas13d_NT crRNA, rep 1 |
| GSM6923687 |
RfxCas13d_K1 crRNA, rep 1 |
| GSM6923688 |
RfxCas13d_K8 crRNA, rep 1 |
| GSM6923689 |
RfxCas13d_M1 crRNA, rep 1 |
| GSM6923690 |
RfxCas13d_M3 crRNA, rep 1 |
| GSM6923691 |
RfxCas13d_T4 crRNA, rep 1 |
| GSM6923692 |
RfxCas13d_T6 crRNA, rep 1 |
| GSM6923693 |
RfxCas13d_N5 crRNA, rep 1 |
| GSM6923694 |
dRfxCas13d, rep 2 |
| GSM6923695 |
dRfxCas13d_NT crRNA, rep 2 |
| GSM6923696 |
dRfxCas13d_K1 crRNA, rep 2 |
| GSM6923697 |
dRfxCas13d_K8 crRNA, rep 2 |
| GSM6923698 |
dRfxCas13d_M1 crRNA, rep 2 |
| GSM6923699 |
dRfxCas13d_M3 crRNA, rep 2 |
| GSM6923700 |
dRfxCas13d_T4 crRNA, rep 2 |
| GSM6923701 |
dRfxCas13d_T6 crRNA, rep 2 |
| GSM6923702 |
dRfxCas13d_N5 crRNA, rep 2 |
| GSM6923703 |
RfxCas13d, rep 2 |
| GSM6923704 |
RfxCas13d_NT crRNA, rep 2 |
| GSM6923705 |
RfxCas13d_K1 crRNA, rep 2 |
| GSM6923706 |
RfxCas13d_K8 crRNA, rep 2 |
| GSM6923707 |
RfxCas13d_M1 crRNA, rep 2 |
| GSM6923708 |
RfxCas13d_M3 crRNA, rep 2 |
| GSM6923709 |
RfxCas13d_T4 crRNA, rep 2 |
| GSM6923710 |
RfxCas13d_T6 crRNA, rep 2 |
| GSM6923711 |
RfxCas13d_N5 crRNA, rep 2 |
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| Relations |
| BioProject |
PRJNA922152 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE222461_N2a_fpkm.xlsx |
6.0 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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