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Series GSE22354 Query DataSets for GSE22354
Status Public on Dec 31, 2010
Title Drosophila expression data from larval, prepupal and pupal wings to identify targets of Hox protein Ultrabithorax (Ubx)
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary Hox genes regionalize the animal body axis by modifying complex morphogenetic and differentiation processes during development. The transformation of wings into halteres by the Hox gene Ultrabithorax (Ubx) in Drosophila melanogaster presents an excellent model system to study the transcriptional networks that control such complex developmental programmes.
We have employed an inducible misexpression system to switch on Ubx in the wing epithelium at successive larval, prepupal and pupal stages, and have used microarray expression profiling to identify the primary transcriptional responses to Ubx. We find that Ubx regulates hundreds of downstream genes, mostly in a subtle manner. These targets are largely distinct at the different stages of appendage development and diversification.
 
Overall design We have generated an experimental fly line combining the nabGal4NP3537-driver, a tub-GAL80ts transgene, and a UAS-UbxIa transgene (the control line was carrying a UAS-eGFP transgene instead). Our core microarray analysis has involved comparison of the transcriptional profile of experimental wings carrying the UAS-UbxIa transgene with that of control wings carrying the UAS-eGFP transgene. Pairwise comparisons have been carried out at three successive developmental stages, in particular at (i) the third instar larval wandering stage, about 4hrs before puparium formation at 29˚C, (ii) the prepupal stage, 6hrs after puparium formation (APF) at 29˚C, and (iii) the early pupal stage, 16hrs APF at 29˚C. Moreover, pairwise comparisons have been carried out with samples developed exclusively at 19˚C (UbxIa or eGFP expression OFF), as well as with samples collected at 16hrs after the temperature shift from 19 to 29˚C (UbxIa or eGFP expression ON). This has allowed us to distinguish the Ubx-dependent effects from the intrinsic expression differences between the fly lines used, and from the temperature-induced responses. We have carried out 4 biological replicates for each condition making a total of 48 hybridizations to Affymetrix Drosophila Genome 2.0 arrays.
 
Contributor(s) Pavlopoulos A, Akam M
Citation(s) 21282633
Submission date Jun 15, 2010
Last update date Aug 28, 2018
Contact name Anastasios Tassos Pavlopoulos
E-mail(s) ap448@cam.ac.uk
Phone 0044 (0)1223 331773
Fax 0044 (0)1223 336679
URL http://www.zoo.cam.ac.uk/zoostaff/mml/akam/members/tassos.htm
Organization name University of Cambridge
Department Department of Zoology
Lab Laboratory for Development and Evolution (Akam group)
Street address Downing Street
City Cambridge
ZIP/Postal code CB2 3EJ
Country United Kingdom
 
Platforms (1)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
Samples (48)
GSM556361 Larva, 19˚C, Ubx, Biol. Repl. 1
GSM556362 Larva, 19˚C, Ubx, Biol. Repl. 2
GSM556363 Larva, 19˚C, Ubx, Biol. Repl. 3
Relations
BioProject PRJNA127497

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22354_RAW.tar 94.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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