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Series GSE223606 Query DataSets for GSE223606
Status Public on Jan 29, 2023
Title Nanostring analysis of medulloblastoma samples using the PanCancer Immune Profile Nanostring Panel
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The present study aimed to characterize the immune checkpoint profile in medulloblastomas, assess whether there is a distinct profile among molecular subgroups, and evaluate its prognostic outcome.
 
Overall design We included 88 formalin-fixed paraffin-embedded (FFPE) medulloblastoma specimens from patients diagnosed at Barretos Cancer Hospital (BCH), Barretos, and Ribeirão Preto Medical School, Brazil, from 1986 to 2018. Experienced pathologists reviewed the histology, and the tumors were previously molecularly characterized by a 22-gene nCounter assay into WNT (n=14), SHH (n=43), Group 3 (n=12), and Group 4 (n=19). Additionally, three non-tumoral samples derived from non-tumor tissue surrounding brain tumor metastasis were included in the study. Gene expression assays were performed in the nCounter® FLEX Analysis System, using the PanCancer Immune Profiling Panel (NanoString Technologies, Inc., Seattle, WA). This panel comprises 730 immuno-oncology-related targets, including 109 cell surface markers for 14 immune cell types and 40 reference genes (https://nanostring.com/products/ncounter-assays-panels/oncology/pancancer-immune-profiling/).
 
Contributor(s) Ferreira Marques RP, Moreno DA, Silva L, Leal LF, de Paula FE, Santana I, Teixeira G, Saggioro F, Neder L, Mançano B, Reis RM, Junior CA
Citation(s) 36825029
Submission date Jan 24, 2023
Last update date Mar 10, 2023
Contact name Rui Pedro Ferreira Marques
E-mail(s) pg45863@alunos.uminho.pt
Organization name School of Medicine, University of Minho
Department Life and Health Sciences Institute
Lab BiTCancer
Street address Campus de Gualtar
City Braga
ZIP/Postal code 4710-057
Country Portugal
 
Platforms (1)
GPL32232 NanoString nCounter PanCancer Immune Profiling Panel (NS_CancerImmune_V1.1)
Samples (83)
GSM6965296 Patient 1
GSM6965297 Patient 2
GSM6965298 Patient 3
Relations
BioProject PRJNA926966

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE223606_GSE_rawcounts.xlsx 297.6 Kb (ftp)(http) XLSX
Processed data included within Sample table

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