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| Status |
Public on Dec 01, 2010 |
| Title |
Early gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation |
| Platform organism |
Canis lupus familiaris |
| Sample organism |
Mustela putorius furo |
| Experiment type |
Expression profiling by array
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| Summary |
Background: Type I interferons (IFNs) are essential to the clearance of viral diseases, in part by initiating upregulation of IFN regulated genes (IRGs). A clear distinction between genes upregulated directly by virus and genes upregulated by secondary IFN production has not been made. Here we investigated the genes regulated by IFN-a2b compared to the genes regulated by SARS-CoV infection in ferrets.
Methods: We characterized early host immune responses in peripheral blood and lung necropsies of ferrets injected with IFN-a2b or infected with SARS-CoV/Tor 2 strain, using microarray analysis on the Affymetrix platform.
Results: We identified a common IRG signature that was upregulated in both SARS-CoV infected ferrets as well as in ferrets injected with IFN-a2b. We also identified unique patterns of gene expression for leukocyte activation, cell adhesion and complement pathways between IFN-a2b injection and SARS-CoV infection.
Conclusions: Our results define the effects of IFN-a2b on the immune system of ferrets highlighting genes regulated by IFN during SARS-CoV infection. We have shown the similarities and differences of top funcional gene groups as well as pathways that play key roles in early immune responses in ferrets in response to IFN-a2b or SARS-CoV.
Key words: ferret, gene expression, SARS, interferon.
Keywords: time course
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| Overall design |
In experiments with IFN-a2b, for peripheral blood, 15 ferrets were randomly allocated to 3 groups: Day 0, 5 ferrets (no IFN injection), day 1, 6 ferrets (injected), and day 2, 4 ferrets (injected). For lung necropsies of injected ferrets with IFN-a2b, we used 12 ferrets in 3 groups: 4 ferrets, day 0 (no IFN injection), 4 ferrets, day 1 (injected) and 4 ferrets, day 2 (injected). Experimental groups for SARS-CoV infection was as follows: For peripheral blood, 3 and 4 ferrets for day 0 (no infection) and day 2 (infection) respectively. For lung neceropsies, a total of 9 ferrets in 3 groups, each with 3 replicates for day 0 (no infection), day 1 (infection) and day 2 (infection).
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| Contributor(s) |
Kelvin DJ |
| Citation(s) |
21035159 |
| Submission date |
Jun 25, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Longsi Ran |
| E-mail(s) |
lran@uhnresearch.ca
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| Phone |
416-581-7607
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| Organization name |
Toronto General Research Institute
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| Department |
Division of Experimental Therapeutics
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| Lab |
Dr. David J Kelvin
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| Street address |
101 College Street
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| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
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| Platforms (1) |
| GPL3738 |
[Canine_2] Affymetrix Canine Genome 2.0 Array |
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| Samples (43)
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| GSM560358 |
Ferret Blood, Not injected with IFN-a2b, on Day 0, biological rep1 |
| GSM560359 |
Ferret Blood, Not injected with IFN-a2b, on Day 0, biological rep2 |
| GSM560360 |
Ferret Blood, Not injected with IFN-a2b, on Day 0, biological rep3 |
| GSM560361 |
Ferret Blood, Not injected with IFN-a2b, on Day 0, biological rep4 |
| GSM560362 |
Ferret Blood, Not injected with IFN-a2b, on Day 0, biological rep5 |
| GSM560363 |
Ferret Blood, injected with IFN-a2b on Day 1, biological rep1 |
| GSM560364 |
Ferret Blood, injected with IFN-a2b on Day 1, biological rep2 |
| GSM560365 |
Ferret Blood, injected with IFN-a2b on Day 1, biological rep3 |
| GSM560366 |
Ferret Blood, injected with IFN-a2b on Day 1, biological rep4 |
| GSM560367 |
Ferret Blood, injected with IFN-a2b on Day 1, biological rep5 |
| GSM560368 |
Ferret Blood, injected with IFN-a2b on Day 1, biological rep6 |
| GSM560369 |
Ferret Blood, injected with IFN-a2b on Day 2, biological rep1 |
| GSM560370 |
Ferret Blood, injected with IFN-a2b on Day 2, biological rep2 |
| GSM560371 |
Ferret Blood, injected with IFN-a2b on Day 2, biological rep3 |
| GSM560372 |
Ferret Blood, injected with IFN-a2b on Day 2, biological rep4 |
| GSM560373 |
Ferret Lung, Not injected with IFN-a2b, on Day 0, biological rep1 |
| GSM560374 |
Ferret Lung, Not injected with IFN-a2b, on Day 0, biological rep2 |
| GSM560375 |
Ferret Lung, Not injected with IFN-a2b, on Day 0, biological rep3 |
| GSM560376 |
Ferret Lung, Not injected with IFN-a2b, on Day 0, biological rep4 |
| GSM560377 |
Ferret Lung, injected with IFN-a2b on Day 1, biological rep1 |
| GSM560378 |
Ferret Lung, injected with IFN-a2b on Day 1, biological rep2 |
| GSM560379 |
Ferret Lung, injected with IFN-a2b on Day 1, biological rep3 |
| GSM560380 |
Ferret Lung, injected with IFN-a2b on Day 1, biological rep4 |
| GSM560381 |
Ferret Lung, injected with IFN-a2b on Day 2, biological rep1 |
| GSM560382 |
Ferret Lung, injected with IFN-a2b on Day 2, biological rep2 |
| GSM560383 |
Ferret Lung, injected with IFN-a2b on Day 2, biological rep3 |
| GSM560384 |
Ferret Lung, injected with IFN-a2b on Day 2, biological rep4 |
| GSM560385 |
Ferret Blood, uninfected, on Day 0, biological rep1 |
| GSM560386 |
Ferret Blood, uninfected, on Day 0, biological rep2 |
| GSM560387 |
Ferret Blood, uninfected, on Day 0, biological rep3 |
| GSM560388 |
Ferret Blood, infecetd with SARS-CoV on Day 2, biological rep1 |
| GSM560389 |
Ferret Blood, infecetd with SARS-CoV on Day 2, biological rep2 |
| GSM560390 |
Ferret Blood, infecetd with SARS-CoV on Day 2, biological rep3 |
| GSM560391 |
Ferret Blood, infecetd with SARS-CoV on Day 2, biological rep4 |
| GSM560392 |
Ferret Lung, uninfected, on Day 0, biological rep1 |
| GSM560393 |
Ferret Lung, uninfected, on Day 0, biological rep2 |
| GSM560394 |
Ferret Lung, uninfected, on Day 0, biological rep3 |
| GSM560395 |
Ferret Lung, infecetd with SARS-CoV on Day 1, biological rep1 |
| GSM560396 |
Ferret Lung, infecetd with SARS-CoV on Day 1, biological rep2 |
| GSM560397 |
Ferret Lung, infecetd with SARS-CoV on Day 1, biological rep3 |
| GSM560398 |
Ferret Lung, infecetd with SARS-CoV on Day 2, biological rep1 |
| GSM560399 |
Ferret Lung, infecetd with SARS-CoV on Day 2, biological rep2 |
| GSM560400 |
Ferret Lung, infecetd with SARS-CoV on Day 2, biological rep3 |
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| Relations |
| BioProject |
PRJNA128405 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22581_RAW.tar |
139.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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