|
| Status |
Public on May 13, 2011 |
| Title |
Tissue Specific Pathways for Estrogen Regulation of Ovarian Cancer Growth and Metastasis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Menopausal estrogen (E2) replacement therapy increases the risk of estrogen receptor (ER)-positive epithelial ovarian cancers (EOC). Whether E2 is tumorigenic or promotes expansion of undiagnosed pre-existing disease is unknown. To determine E2 effects on tumor promotion, we developed an intraperitoneal mouse xenograft model using ZsGreen fluorescent ER- 2008 and ER+ PEO4 human EOC cells. Tumor growth was quantified by in vivo fluorescent imaging. In ER+ tumors, E2 significantly increased size, induced progesterone receptors, and promoted lymph node metastasis, confirming that ER are functional and foster aggressiveness. Laser captured human EOC cells from ER- and ER+ xenografted tumors were profiled for expression of E2-regulated genes. Three classes of E-regulated EOC genes were defined, but less than 10% were shared with E-regulated breast cancer genes. Since breast cancer selective ER modulators (SERM) are therapeutically ineffective in EOC, we suggest that our EOC-specific E-regulated genes can assist pharmacologic discovery of ovarian targeted SERM.
|
| |
|
| Overall design |
15 samples were included in this experiment with a 2x2 factorial design with 2 different cell lines (2008 and PEO4) and 2 different hormone treatments (E for Estrogen and C for Placebo Control) and 4 replicates per treatment. 1 sample was excluded (a replicate of PEO4 with C treatment) because of poor quality.
|
| |
|
| Contributor(s) |
Spillman MA, Manning NG, Dye WW, Sartorius CA, Post MD, Harrell C, Jacobsen BM, Horwitz KB |
| Citation(s) |
20959477 |
| Submission date |
Jun 28, 2010 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Monique Spillman |
| E-mail(s) |
Monique.Spillman@ucdenver.edu
|
| Organization name |
University of Colorado Denver
|
| Street address |
Mail Stop B198-4, PO Box 6511
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA128381 |
Less...
More...