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Series GSE22611 Query DataSets for GSE22611
Status Public on Jan 01, 2013
Title NOD2 and desease associated variant NOD2-L1007fsinsC dependent genomewide transcriptional regulation in stable Flp-In HEK cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary NOD2 is an intracellular receptor for the bacterial cell wall component muramyl dipeptide (MDP) and variants of NOD2 are associated with chronic inflammatory diseases of barrier organs e.g. Crohn disease, asthma and atopic eczema. It is known that activation of NOD2 induces a variety of inflammatory and antibacterial factors. The exact transcriptomal signatures that define the cellular programs downstream of NOD2 activation and the influence of the Crohn-associated variant L1007fsinsC are yet to be defined. To describe the MDP-induced activation program, we analyzed the transcriptomal reactions of isogenic HEK293 cells expressing NOD2wt or NOD2L1007fsinsC to stimulation with MDP. Importantly, a clear loss-of-function could be observed in the cells carrying the Crohn-associated variant L1007fsinsC, while the NOD2wt cells showed differential regulation of growth factors, chemokines and several antagonists of NF-κB, e.g. TNFAIP3 (A20) and IER3.
 
Overall design To elucidate the MDP-induced activation program we generated isogenic HEK293 cells stably expressing wildtype NOD2 or NOD2L1007fsinsC using a FRT-recombinase based approach. Cells carrying the inserted vector cassette were used as controls (mock-transfectant). To comprehensively analyze NOD2-mediated innate immune responses we analyzed transcriptomal signature patterns using genome-wide cDNA microarrays. Samples were harvested from cell cultures under normal growth conditions 0 h, 2 h and 6 h after MDP‑ stimulation of the cells.
 
Contributor(s) Billmann S, Till A, Haesler R, Kerick M, Hanidu A, Li J, Rosenstiel P, Schreiber S
Citation(s) 21335489
Submission date Jun 29, 2010
Last update date Mar 25, 2019
Contact name Susanne Billmann
Organization name Institute of Clinical Molecular Biology Christian-Albrechts-Universität Kiel
Street address Schittenhelmstraße 12
City Kiel
ZIP/Postal code D-24105
Country Germany
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (27)
GSM560855 NOD2wt_0h_MDP_biological replicate 1
GSM560856 NOD2wt_0h_MDP_biological replicate 2
GSM560857 NOD2wt_0h_MDP_biological replicate 3
Relations
BioProject PRJNA128351

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22611_RAW.tar 216.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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