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| Status |
Public on Dec 31, 2023 |
| Title |
Gene expression profiles associated with myxoid liposarcoma scaffold culture compared to monolayer growth and effect of fusion oncogene FUS-DDIT3 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Myxoid liposarcoma (MLS) is the second most common type of liposarcoma and is characterized by the fusion oncogene FUS‐DDIT3 or the less common EWSR1‐DDIT3. FUS-DDIT3 is causative in tumor development, but the molecular function of FUS-DDIT3 remains largely unknown. In addition, the tumor microenvironment is important in MLS development. However, due to a lack of relevant experimental model systems, it has been challenging to examine the microenvironmental impact in MLS development. Therefore, we have developed an in vivo-like experimental model system utilizing cell-free scaffolds derived from myxoid liposarcoma patient-derived xenograft tumors that can be repopulated with tumor cells. To study the effect of FUS-DDIT3 expression in combination with the MLS microenvironment, we analyzed MLS cell lines as well as fibrosarcoma cells with and without ectopic FUS-DDIT3 expression cultured in scaffolds using cells cultured in monolayers as reference. We identified several gene networks and processes that are uniquely associated with FUS-DDIT3 expression as well as microenvironment. The use of in vivo-like experimental systems opens new possibilities to understand tumor development and develop treatments.
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| Overall design |
Five cell lines were cultured in scaffolds and monolayer (2D) in 3-5 replicates and analyzed using RNA sequencing: Two MLS cell lines, 1765-92 and 2645-94, as well as HT1080 fibrosarcoma cells with and without fusion oncogene FUS-DDIT3 including native HT1080 cells, HT1080 cells expressing EGFP and HT1080 cells expressing FUS-DDIT3-EGFP.
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| Contributor(s) |
Ranji P, Jonasson E, Andersson L, Filges S, Luna Santamaría M, Dolatabadi S, Åman P, Ståhlberg A |
| Citation(s) |
38671504 |
| Submission date |
Apr 27, 2023 |
| Last update date |
May 08, 2024 |
| Contact name |
Emma Jonasson |
| Organization name |
University of Gothenburg
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| Department |
Sahlgrenska Center for Cancer Research
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| Street address |
Medicinaregatan 1F, floor 5
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| City |
Göteborg |
| ZIP/Postal code |
41390 |
| Country |
Sweden |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL22790 |
Illumina MiniSeq (Homo sapiens) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA962554 |
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