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| Status |
Public on May 07, 2023 |
| Title |
Diurnal changes in bacterial settlement on the Peyer’s patch and surrounding mucosa in the rat ileum and its effect against the intestinal immune system |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate how stimulation from indigenous bacteria for a day affects the local transcriptome of both Peyer's patch and surrounding ordinary mucosa in the rat ileum, we treated rats with a nonabsorbable antibiotics cocktail (vancomycin, polymyxin B and sucrose) for antibiotics-treatment group or sucrose solution for control group in drinking water 1 day before euthanasia.
We then performed gene expression profiling analysis using data obtained from RNA-seq of ileal Peyer's patch and surrounding ordinary tissue from rats with or without antibiotics treatment.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data from Peyer's patch and ordinary ileal tissue between control group and antibiotics treated group.
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| Contributor(s) |
MANTANI Y, KUBOTA N |
| Citation(s) |
37148397 |
| Submission date |
May 02, 2023 |
| Last update date |
Aug 17, 2023 |
| Contact name |
Youhei MANTANI |
| E-mail(s) |
mantani@sapphire.kobe-u.ac.jp
|
| Organization name |
Kobe University
|
| Department |
Graduate school of Agricultural Science
|
| Street address |
Rokkodai-cho 1-1, Nada-ku
|
| City |
Kobe |
| State/province |
Hyogo |
| ZIP/Postal code |
657-8501 |
| Country |
Japan |
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| Platforms (1) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (12)
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| GSM7286115 |
rat ileum, antibiotics treatment [A1_I] |
| GSM7286116 |
rat ileal Peyer's patch, antibiotics treatment [A1_P] |
| GSM7286117 |
rat ileum, antibiotics treatment [A2_I] |
|
| Relations |
| BioProject |
PRJNA966073 |
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