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| Status |
Public on Mar 27, 2024 |
| Title |
Multiple parallel cell lineages in the developing mammalian cerebral cortex |
| Organisms |
Mustela putorius; Mustela putorius furo |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Cortical neurogenesis follows a simple lineage: apical Radial Glia Cells (RGC) generate basal progenitors, and these produce neurons. How this occurs in species with expanded germinal zones and a folded cortex, like human, remains unclear. We used single-cell RNA sequencing from individual cortical germinal zones in ferret, and barcoded lineage tracking, to determine the molecular diversity of progenitor cells and their lineages. We identified multiple RGC classes that initiate parallel lineages, converging onto a common class of newborn neuron. Parallel RGC classes and transcriptomic trajectories were repeated across germinal zones and conserved in ferret and human, but not mouse. Neurons followed parallel differentiation trajectories in gyrus and sulcus, with different expression of human cortical malformation genes. Progenitor cell lineage multiplicity is conserved in the folded mammalian cerebral cortex.
This SuperSeries is composed of the SubSeries listed below.
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| |
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| Overall design |
Refer to individual Series
Single-cell RNA sequencing (Borrell lab):
https://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/geo/query/acc.cgi?acc=GSE219254
Barcoded lineage tracking (Mayer lab):
https://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/geo/query/acc.cgi?acc=GSE233587
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| Web link |
https://0-doi-org.brum.beds.ac.uk/10.1126/sciadv.adn9998
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| |
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| Citation(s) |
38536915 |
| Submission date |
Jun 06, 2023 |
| Last update date |
Apr 04, 2024 |
| Contact name |
Lucia Del-Valle-Anton |
| Organization name |
Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas & Universidad Miguel Hernández
|
| Lab |
Borrell Lab
|
| Street address |
Av. Santiago Ramón y Cajal s/n
|
| City |
Sant Joan d’Alacant |
| ZIP/Postal code |
03550 |
| Country |
Spain |
| |
|
| Platforms (2) |
| GPL28629 |
Illumina NovaSeq 6000 (Mustela putorius furo) |
| GPL32908 |
Illumina HiSeq 2500 (Mustela putorius) |
|
| Samples (26)
|
| GSM6779220 |
E34_VZ_LS, rep1 |
| GSM6779221 |
E34_VZ_LS, rep2 |
| GSM6779222 |
E34_VZ_LS, rep3 |
| GSM6779223 |
P1_VZ_SG, rep1 |
| GSM6779224 |
P1_VZ_SG, rep2 |
| GSM6779225 |
P1_VZ_SG, rep3 |
| GSM6779226 |
P1_VZ_LS, rep1 |
| GSM6779227 |
P1_VZ_LS, rep2 |
| GSM6779228 |
P1_VZ_LS, rep3 |
| GSM6779229 |
P1_OSVZ_SG, rep1 |
| GSM6779230 |
P1_OSVZ_SG, rep2 |
| GSM6779231 |
P1_OSVZ_SG, rep3 |
| GSM6779232 |
P1_OSVZ_LS, rep1 |
| GSM6779233 |
P1_OSVZ_LS, rep2 |
| GSM6779234 |
P1_OSVZ_LS, rep3 |
| GSM7430897 |
Transcriptomic dataset of sample 1 |
| GSM7430898 |
Transcriptomic dataset of sample 2 |
| GSM7430899 |
Transcriptomic dataset of sample 3 |
| GSM7430900 |
Transcriptomic dataset of sample 4 |
| GSM7430901 |
Barcode dataset of sample 1 |
| GSM7430902 |
Barcode dataset of sample 2 |
| GSM7430903 |
Barcode dataset of sample 3 |
| GSM7430904 |
Barcode dataset of sample 4 |
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| This SuperSeries is composed of the following SubSeries: |
| GSE219254 |
scRNA-seq of ferret cortical germinal zones from splenial gyrus and lateral sulcus (Borrell lab) |
| GSE233587 |
Barcode lineage tracking of ferret cortical progeny using TrackerSeq (Mayer lab) |
|
| Relations |
| BioProject |
PRJNA980720 |
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