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| Status |
Public on Nov 17, 2023 |
| Title |
Integrative single-cell transcriptome analysis of human pancreatic cancer reveals an intermediate cancer cell population associated with poor prognosis [Spatial] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
We identified five distinct functional subclusters of pancreatic cancer cells and six distinct cancer-associated fibroblast subclusters. We deeply profiled their characteristics, and we found that these subclusters successfully deconvoluted most of the features suggested in bulk transcriptome analysis of pancreatic cancer. Among those subclusters, we identified a novel cancer cell subcluster, Ep_VGLL1, showing intermediate characteristics between the extremities of basal-like and classical dichotomy, despite its prognostic value. Molecular features of Ep_VGLL1 suggest its transitional properties between basal-like and classical subtypes, which is strongly supported by spatial transcriptome data.
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| Overall design |
We performed single cell RNA sequencing using enriched non-immune cell populations from 17 pancreatic cancer tumor tissues, and also generated paired spatial transcriptome data from 7 of the patient samples.
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| Contributor(s) |
Leem G, Choi J, Kim S |
| Citation(s) |
38297291 |
| Submission date |
Jun 20, 2023 |
| Last update date |
Feb 13, 2024 |
| Contact name |
Jong-Eun Park |
| Organization name |
Korea Advanced Institute of Science and Technology
|
| Street address |
291, Daehak-ro, Yuseong-gu
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| City |
Daejeon |
| ZIP/Postal code |
34141 |
| Country |
South Korea |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE235452 |
Integrative single-cell transcriptome analysis of human pancreatic cancer reveals an intermediate cancer cell population associated with poor prognosis |
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| Relations |
| BioProject |
PRJNA985689 |
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