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| Status |
Public on Mar 31, 2024 |
| Title |
Monoubiquitination of histone H2B is a master regulator of the transcriptome during memory formation |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report a vital role for H2Bubi in active and repressive gene transcription during long-term memory formation. We found that fear conditioning increased or decreased the expression of 86 genes in the hippocampus one hour after training. Remarkably, siRNA knockdown of the H2Bubi ligase, Rnf20, abolished changes in all but one of these genes, Per1, suggesting a critical role for the H2Bubi epigenetic mark in active and repressive gene transcription during memory consolidation.
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| Overall design |
Male Sprague-Dawley rats were injected into the dorsal hippocampus CA1 region with Accell siRNA against Rnf20 or control (Scr-siRNA). Five days later animals were trained to contextual fear conditioning and the CA1 region (dorsal) was dissected, RNA collected via columns and used for RNA-seq analysis
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| Contributor(s) |
Navabpour S, Farrell K, Omar N, Musaus M, Lin Y, Xie H, Jarome TJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 22, 2023 |
| Last update date |
Mar 31, 2024 |
| Contact name |
Timothy Jarome |
| E-mail(s) |
tjjarome@vt.edu
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| Organization name |
Virginia Polytechnic Institute and State University
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| Street address |
175 W Campus Dr
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| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24061 |
| Country |
USA |
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| Platforms (1) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA986551 |
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