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Series GSE237798 Query DataSets for GSE237798
Status Public on Jul 23, 2023
Title Combined phenotypic and transcriptomic analysis of soybean plants subjected to multifactorial stress combination
Organism Glycine max
Experiment type Expression profiling by high throughput sequencing
Summary Soybean plants were subjected to a multifactorial stress combination of up to five different stresses (water deficit, salinity, low phosphate, acidity, and cadmium), in an increasing level of complexity. All stresses were applied at the beginning of the experiment except for water deficit stress that was imposed after 21 days. Leaves and flowers were collected from 5-7 different plants under the mentioned stress conditions and after 10 days of starting water deficit conditions. Differential gene expression compared to control was studied using RNAseq method for all the possible stress combinations.
 
Overall design Tissues from soybean plants (31 days old) subjected to water deficit, salinity, low phosphate, acidity, and cadmium alone or in combination of 2, 3, 4 and 5 stresses were collected in liquid nitrogen. Total RNA was isolated and mRNA profile was generated by deep sequencing using NovaSeq 6000 PE150.
Web link https://0-doi-org.brum.beds.ac.uk/10.1101/2023.08.15.553447
 
Contributor(s) Peláez-Vico MA, Induri SP, Sinha R, Shostak B, Lyu Z, Venigalla SD, Vasireddy D, Singh P, Immadi MS, Pascual LS, Cozatl DM, Joshi T, Fritschi FB, Zandalinas SI, Mittler R
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Submission date Jul 19, 2023
Last update date Oct 25, 2023
Contact name Ron Mittler
E-mail(s) mittlerr@missouri.edu
Organization name The Division of Plant Sciences and Technology
Department Christopher S. Bond Life Sciences Center
Lab LSC 312
Street address University of Missouri,1201 Rollins St
City Columbia
State/province Missouri
ZIP/Postal code 65201
Country USA
 
Platforms (1)
GPL22410 Illumina NextSeq 500 (Glycine max)
Samples (108)
GSM7653526 Control soybean leaves replicate 1
GSM7653527 Control soybean leaves replicate 2
GSM7653528 Control soybean leaves replicate 3
Relations
BioProject PRJNA996630

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE237798_flower_fpkm.txt.gz 8.6 Mb (ftp)(http) TXT
GSE237798_leaf_fpkm.txt.gz 8.0 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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