|
| Status |
Public on Jan 12, 2024 |
| Title |
Single-nucleus RNA-sequencing of human subcutaneous adipose tissue biopsies from 84 Finnish participants. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed single-nucleus RNA-seq using 10X on 84 randomly sampled participants from the METabolic Syndrome In Men (METSIM) cohort. The purpose of the study was to characterize cell type composition changes associated with metabolic diseases such as obesity and type 2 diabetes.
|
| |
|
| Overall design |
Single cell RNA-seq samples were multiplexed to increase sample size. Fresh-frozen subcutaneous adipose tissue biopsies from 4 donors were pooled immediately prior to isolation. We then isolated nuclei using mincing, washing, and straining. Single nucleus sequencing libraries were prepared using the 10X single cell 3' v3.1 kit and the 10X Chromium platform. The resulting libraries were sequenced on an Illumina NovaSeq targeting 200 million reads per sample. Samples were demultiplexed with Demuxlet.
***************************************************************
Submitter states that missing raw files are due to patient privacy concerns.
***************************************************************
|
| |
|
| Contributor(s) |
Alvarez M, Pajukanta P, Laakso M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 30, 2023 |
| Last update date |
Jan 12, 2024 |
| Contact name |
Marcus Alvarez |
| E-mail(s) |
malvarez@mednet.ucla.edu
|
| Organization name |
UCLA
|
| Department |
Human Genetics
|
| Lab |
Pajukanta
|
| Street address |
695 Charles E Young Dr South, Gonda 6335
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (84)
|
|
| Relations |
| BioProject |
PRJNA1047144 |
Less...
More...