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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 04, 2011 |
| Title |
Gene expression profiling of skeletal muscles treated with a soluble activin type IIB receptor |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Inhibition of the myostatin signaling pathway is emerging as a promising therapeutic means to treat muscle wasting disorders. Activin type IIB receptor is the putative myostatin receptor, and a soluble activin receptor (ActRIIB-Fc) has been demonstrated to potently inhibit a subset of TGF-β family members including myostatin. In order to determine reliable and valid biomarkers for myostatin pathway inhibition, we assessed gene expression profiles for quadriceps muscles from mice treated with ActRIIB-Fc compared to mice genetically lacking myostatin and control mice.
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| Overall design |
RNA extracted from quadriceps muscles of four classes of two-month-old female mice were analyzed with Affymetrix microarrays: control mice, myostatin-null mice, mice treated with one dose of ActRIIB-Fc and mice treated with four doses of ActRIIB-Fc. An initial study used 3 mice from each class, and an independent follow-up study used 8 myostatin-null mice and 5 mice from each of the other 3 classes.
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| Contributor(s) |
Rahimov F |
| Citation(s) |
21266502 |
| Submission date |
Nov 12, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Fedik Rahimov |
| E-mail(s) |
frahimov@enders.tch.harvard.edu
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| Phone |
617-355-4042
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| Fax |
617-730-0253
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| Organization name |
Boston Children's Hospital
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| Department |
Genetics
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| Lab |
Kunkel
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| Street address |
3 Blackfan Circle, CLS 15030.4
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (35)
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| GSM645150 |
quadriceps, control mouse, experiment1, biological rep1 |
| GSM645151 |
quadriceps, control mouse, experiment1, biological rep2 |
| GSM645152 |
quadriceps, control mouse, experiment1, biological rep3 |
| GSM645153 |
quadriceps, mouse treated with four doses of ActRIIB-Fc, experiment1, biological rep1 |
| GSM645154 |
quadriceps, mouse treated with four doses of ActRIIB-Fc, experiment1, biological rep2 |
| GSM645155 |
quadriceps, mouse treated with four doses of ActRIIB-Fc, experiment1, biological rep3 |
| GSM645156 |
quadriceps, mstn-null mouse, experiment1, biological rep1 |
| GSM645157 |
quadriceps, mstn-null mouse, experiment1, biological rep2 |
| GSM645158 |
quadriceps, mstn-null mouse, experiment1, biological rep3 |
| GSM645159 |
quadriceps, mouse treated with one dose of ActRIIB-Fc, experiment1, biological rep1 |
| GSM645160 |
quadriceps, mouse treated with one dose of ActRIIB-Fc, experiment1, biological rep2 |
| GSM645161 |
quadriceps, mouse treated with one dose of ActRIIB-Fc, experiment1, biological rep3 |
| GSM645162 |
quadriceps, control mouse, experiment2, biological rep1 |
| GSM645163 |
quadriceps, control mouse, experiment2, biological rep2 |
| GSM645164 |
quadriceps, control mouse, experiment2, biological rep3 |
| GSM645165 |
quadriceps, control mouse, experiment2, biological rep4 |
| GSM645166 |
quadriceps, control mouse, experiment2, biological rep5 |
| GSM645167 |
quadriceps, mouse treated with four doses of ActRIIB-Fc, experiment2, biological rep1 |
| GSM645168 |
quadriceps, mouse treated with four doses of ActRIIB-Fc, experiment2, biological rep2 |
| GSM645169 |
quadriceps, mouse treated with four doses of ActRIIB-Fc, experiment2, biological rep3 |
| GSM645170 |
quadriceps, mouse treated with four doses of ActRIIB-Fc, experiment2, biological rep4 |
| GSM645171 |
quadriceps, mouse treated with four doses of ActRIIB-Fc, experiment2, biological rep5 |
| GSM645172 |
quadriceps, mstn-null mouse, experiment2, biological rep1 |
| GSM645173 |
quadriceps, mstn-null mouse, experiment2, biological rep2 |
| GSM645174 |
quadriceps, mstn-null mouse, experiment2, biological rep3 |
| GSM645175 |
quadriceps, mstn-null mouse, experiment2, biological rep4 |
| GSM645176 |
quadriceps, mstn-null mouse, experiment2, biological rep5 |
| GSM645177 |
quadriceps, mstn-null mouse, experiment2, biological rep6 |
| GSM645178 |
quadriceps, mstn-null mouse, experiment2, biological rep7 |
| GSM645179 |
quadriceps, mstn-null mouse, experiment2, biological rep8 |
| GSM645180 |
quadriceps, mouse treated with one dose of ActRIIB-Fc, experiment2, biological rep1 |
| GSM645181 |
quadriceps, mouse treated with one dose of ActRIIB-Fc, experiment2, biological rep2 |
| GSM645182 |
quadriceps, mouse treated with one dose of ActRIIB-Fc, experiment2, biological rep3 |
| GSM645183 |
quadriceps, mouse treated with one dose of ActRIIB-Fc, experiment2, biological rep4 |
| GSM645184 |
quadriceps, mouse treated with one dose of ActRIIB-Fc, experiment2, biological rep5 |
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| Relations |
| BioProject |
PRJNA134795 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE25306_RAW.tar |
138.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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