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| Status |
Public on May 06, 2024 |
| Title |
Antagonistic conflict between transposon-encoded introns and guide RNAs (RNA-Seq) |
| Organisms |
Escherichia coli; Clostridium senegalense |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
TnpB nucleases represent the evolutionary precursors to CRISPR-Cas12 and are widespread in all domains of life. IS605-family TnpB homologs function in bacteria as programmable RNA-guided homing endonucleases driving transposon maintenance through DSB-stimulated homologous recombination. Here we uncover molecular mechanisms of transposition lifecycle of IS607-family elements that, remarkably, also encode group I introns. We discover molecular features for a candidate ‘IStron’ from Clostridium botulinum that allow the element to carefully control the relative levels of spliced products versus functional guide RNAs. Our results suggest that IStron transcripts have evolved a sensitive equilibrium to balance competing and mutually exclusive activities that promote transposon maintenance while limiting adverse fitness costs on the host. Collectively, this work highlights molecular innovation in the multi-functional utility of transposon-encoded noncoding RNAs.
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| Overall design |
E. coli total RNA-seq profiles for CboIStron and CseIStrons, as well as total RNA-seq of C. senegalense.
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| |
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| Contributor(s) |
Žedaveinytė R, Meers C, Le HC, Mortman EE, Tang S, Lampe GD, Pesari SR, Gelsinger DR, Wiegand T, Sternberg SH |
| Citation missing |
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| |
| Submission date |
Mar 11, 2024 |
| Last update date |
May 07, 2024 |
| Contact name |
Samuel Henry Sternberg |
| E-mail(s) |
shsternberg@gmail.com
|
| Phone |
717-475-3658
|
| Organization name |
Columbia University
|
| Department |
Biochemistry and Molecular Biophysics
|
| Lab |
Sternberg Lab
|
| Street address |
701 W. 168th Street, HHSC 726
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL21222 |
Illumina NextSeq 500 (Escherichia coli) |
| GPL34292 |
Illumina NextSeq 500 (Clostridium senegalense) |
|
| Samples (4)
|
| GSM8140824 |
C. senegalense total RNA-seq, native |
| GSM8140825 |
C. botulinum IStron total RNA-seq, heterologous |
| GSM8140826 |
C. senegalense IStron-1 total RNA-seq, heterologous |
| GSM8140827 |
C. senegalense IStron-2 total RNA-seq, heterologous |
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| This SubSeries is part of SuperSeries: |
| GSE261344 |
Antagonistic conflict between transposon-encoded introns and guide RNAs |
|
| Relations |
| BioProject |
PRJNA1086528 |
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