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| Status |
Public on Feb 02, 2011 |
| Title |
Comparable mRNA expression of pluripotency-related genes in SNUhES3 cells cultured on autofeeder or xenofeeder system |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
I developed a new culture system for hES cells; this system does not require supplementation with bFGF to obtain hES cells that are suitable for tissue engineering and regenerative medicine. This culture system employed mesenchymal stem cells derived from hES cells (hESC-MSCs) as autologous human feeder cells in the absence of bFGF. For pluripotency-related gene expression profiling, a cDNA microarray analysis was performed
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| Overall design |
SNUhES3 cultured on the autofeeder hESC-MSCs layer maintained the undifferentiated state for 30 passages in a manner similar to the SNUhES3 cells on xenofeeder STO cell layer. To compare the pluripotency-related genes in SNUhES3 cells cultured on autofeeder or xenofeeder system, I used agilent one-color array. Two independant experiment performed.
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| Contributor(s) |
Lee E |
| Citation missing |
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| Submission date |
Feb 01, 2011 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Eun Ju Lee |
| E-mail(s) |
leeunju@snu.ac.kr
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| Phone |
82220721707
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| Organization name |
seoul natonal university
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| Street address |
28 yongon dong
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| City |
seoul |
| ZIP/Postal code |
110744 |
| Country |
South Korea |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA136933 |
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