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| Status |
Public on May 21, 2012 |
| Title |
Genome wide transcriptional analysis of P. aeruginosa PAO1 response to kappa-opioid U-50,488 in poor nutrient medium |
| Platform organism |
Pseudomonas aeruginosa |
| Sample organism |
Pseudomonas aeruginosa PAO1 |
| Experiment type |
Expression profiling by array
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| Summary |
Here we examined virulence activation in Pseudomonas aeruginosa in response to the synthetic kappa opioid agonist U-50, 488 in nutrient poor media where growth conditions are limited and density dependent quorum sensing is not activated.
We used a microarray to define the virulence-related genes in P. aeruginosa grown in liquid poor nutrient medium to determine the effect of kappa-opioid receptor agonist on virulence/lethal phenotype
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| Overall design |
All biological samples for gene expression analysis were prepared in triplicate. P. aeruginosa MPAO1 cells collected from liquid culture at 7 hrs of growth in 1.) 0.1xTY medium containing tryptone 1 g/L and yeast extract, 0.5 g/L, or 2.) 0.1xTY+25 mM potassium phosphate buffer, pH 6.0, or 3.) 0.1xTY+200 uM U-50,488, or 4.) 0.1xTY+ 25 mM potassium phosphate buffer, pH 6.0+ 200 uM U-50,488 were used for RNA isolation. Microarray analysis was performed using Affymetrix P. aeruginosa GeneChips (Affymetrix, Santa Clara, CA) at the University of Chicago Functional Genomics Facility
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| Contributor(s) |
Alverdy J, Zaborina O |
| Citation(s) |
22514685 |
| Submission date |
Jun 13, 2011 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Olga Y ZABORINA |
| E-mail(s) |
ozaborin@surgery.bsd.uchicago.edu
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| Phone |
773-702-3739
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| Fax |
773-834-4546
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| Organization name |
University of Chicago
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| Department |
Surgery
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| Street address |
5841 S Maryland
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL84 |
[Pae_G1a] Affymetrix Pseudomonas aeruginosa Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA140783 |
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