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| Status |
Public on Nov 01, 2011 |
| Title |
The human Ewing's Sarcoma oncoprotein EWS-FLI1 causes Ewing's-type tumors in zebrafish |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
The fusion oncoprotein EWS-FLI1 arises from a t(11;22)(q24;q12) chromosomal translocation and causes Ewing's Sarcoma, a malignant bone tumor. The mechanism whereby EWS-FLI1 transforms cells is unknown. Somatic, mosaic expression of human EWS-FLI1 in zebrafish from the heat shock promoter [Tg(HSP:EWS-FLI1)] caused small round blue cell tumors (SRBCTs) similar to human Ewing's sarcoma.
We performed microarray studies comparing zebrafish SRBCTs to another tumor type, zebrafish malignant peripheral nerve sheath tumors (MPNSTs). The results identify a conserved set of EWS-FLI1-regulated genes,and provide insight into the pathogenesis of Ewing's Sarcoma tumors.
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| Overall design |
Zebrafish SRBCTs arising from somatic insertions of the EWS-FLI1 transgene were collected. MPNSTs from non-transgenic fish of the same genetic background were collected in parallel. RNA was prepared from all samples and hybridized to zebrafish-specific Affymetrix arrays.
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| Contributor(s) |
Leacock S, Amatruda JF |
| Citation(s) |
21979944 |
| Submission date |
Aug 03, 2011 |
| Last update date |
Jan 25, 2018 |
| Contact name |
James F Amatruda |
| E-mail(s) |
james.amatruda@utsouthwestern.edu
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| Phone |
214-648-3896
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| Organization name |
University of Texas Southwestern Medical Center
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| Department |
Pediatrics and Molecular Biology
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| Street address |
5323 Harry Hines Boulevard
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| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-8534 |
| Country |
USA |
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| Platforms (1) |
| GPL1319 |
[Zebrafish] Affymetrix Zebrafish Genome Array |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE31217 |
A zebrafish transgenic model of Ewing's Sarcoma reveals conserved mediators of EWS-FLI1 tumorigenesis |
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| Relations |
| BioProject |
PRJNA154079 |
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