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Series GSE31186 Query DataSets for GSE31186
Status Public on Nov 01, 2011
Title The human Ewing's Sarcoma oncoprotein EWS-FLI1 causes Ewing's-type tumors in zebrafish
Organism Danio rerio
Experiment type Expression profiling by array
Summary The fusion oncoprotein EWS-FLI1 arises from a t(11;22)(q24;q12) chromosomal translocation and causes Ewing's Sarcoma, a malignant bone tumor. The mechanism whereby EWS-FLI1 transforms cells is unknown. Somatic, mosaic expression of human EWS-FLI1 in zebrafish from the heat shock promoter [Tg(HSP:EWS-FLI1)] caused small round blue cell tumors (SRBCTs) similar to human Ewing's sarcoma.
We performed microarray studies comparing zebrafish SRBCTs to another tumor type, zebrafish malignant peripheral nerve sheath tumors (MPNSTs). The results identify a conserved set of EWS-FLI1-regulated genes,and provide insight into the pathogenesis of Ewing's Sarcoma tumors.
Overall design Zebrafish SRBCTs arising from somatic insertions of the EWS-FLI1 transgene were collected. MPNSTs from non-transgenic fish of the same genetic background were collected in parallel. RNA was prepared from all samples and hybridized to zebrafish-specific Affymetrix arrays.
Contributor(s) Leacock S, Amatruda JF
Citation(s) 21979944
Submission date Aug 03, 2011
Last update date Jan 25, 2018
Contact name James F Amatruda
Phone 214-648-3896
Organization name University of Texas Southwestern Medical Center
Department Pediatrics and Molecular Biology
Street address 5323 Harry Hines Boulevard
City Dallas
State/province TX
ZIP/Postal code 75390-8534
Country USA
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (6)
GSM773085 SRBCT biological rep1
GSM773086 SRBCT biological rep2
GSM773087 SRBCT biological rep3
This SubSeries is part of SuperSeries:
GSE31217 A zebrafish transgenic model of Ewing's Sarcoma reveals conserved mediators of EWS-FLI1 tumorigenesis
BioProject PRJNA154079

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31186_RAW.tar 19.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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