|
| Status |
Public on Sep 15, 2011 |
| Title |
Comparative Genomic Hybridization of S. cerevisiae industrial and laboratory strains |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome variation profiling by genome tiling array
|
| Summary |
The environmental stresses and inhibitors encounted by Saccharomyces cerevisiae strains are main limiting factors in bioethanol fermentation. Investigation of the molecular mechanisms underlying the stresses-related phenotypes diversities within and between S. cerevisiae populations could guide the construction of yeast strains with improved stresses tolerance and fermentation performances. Here, we explored the genetic characteristics of the bioethanol S. cerevisiae strains, and elucidated the genetic variations correlated with its advantaged traits (higher ethanol yield under sever conditions and better tolerance to multiple stresses compared to an S288c derived laboratory strain BYZ1). Firstly, pulse-field gel electrophoresis combined with array-comparative genomic hybridization was used to compare the genome structure of industrial strains and the laboratory strain BYZ1.
|
| |
|
| Overall design |
Yeast cells were cultured in YPD medium. Genome DNA was isolated and sonicated. The average length of DNA fragments was 200-1000bp. The shearing DNA was labeled with Cy5/Cy3 and hybridized to NimbleGen S.cerevisiae Whole-Genome Tiling arrays, which is single array design containing all chromosomes with 32bp median probe spacing and totally covered by ~385,000 probes. Scanning was performed with the Axon GenePix 4000B microarray scanner.
YJS329 vs BYZ1
ZK2 vs BYZ1
|
| |
|
| Contributor(s) |
Zheng D, Wu X, Wang P, Li Y |
| Citation(s) |
22978491, 23344998 |
| Submission date |
Sep 03, 2011 |
| Last update date |
Nov 21, 2014 |
| Contact name |
Daoqiong Zheng |
| E-mail(s) |
zhengdaoqiong@163.com
|
| Phone |
86 571 88206636
|
| Organization name |
College of Life Sciences, Zhejiang University
|
| Department |
Institute of Microbiology
|
| Street address |
866 Yuhangtang Road
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310058 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL7699 |
NimbleGen 385k S. cerevisiae 32bp tiling array |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA145241 |
