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| Status |
Public on Sep 13, 2012 |
| Title |
Gene expression profiling of N-acetylglucosaminidase (NAGLU)-depleted HeLa cells versus non-depleted cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We generated a HeLa cell model of mucopolysaccharidosis IIIB (MPSIIIB) by depleting NAGLU. MPSIIIB-associated cell defects were prominent in NAGLU-depleted cells.
We explored alterations of metabolic pathways in NAGLU-depleted cells versus non-depleted control cells by performing gene expression profiling. Exon array transcriptome analysis showed 96 transcripts with increased expression level and 38 transcripts with decreased expression level in NAGLU-depleted versus non-depleted cells.
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| Overall design |
Total RNA was extracted from two independent cultures of non-depleted cells and NAGLU-depleted cells. We considered a minimal fold change of 1.5 fold and a corrected P value lower than 0.05.
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| Contributor(s) |
Roy E, Flamant P, Heard JM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 15, 2011 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Pierre de la Grange |
| E-mail(s) |
pierre.delagrange@genosplice.com
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| Organization name |
GenoSplice technology
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| Department |
Paris Biotech Santé
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| Street address |
29, rue du Faubourg Saint-Jacques
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| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
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| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA147649 |
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