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Series GSE34154 Query DataSets for GSE34154
Status Public on Sep 08, 2012
Title High-fat diet induced gene expression changes in mouse retina may be prevented by introducing bilberries in diet
Organism Mus musculus
Experiment type Expression profiling by array
Summary Obesity and diabetes associated visual impairment and vascular dysfunctions are increasing reasons for vision loss. The detailed mechanisms in these diseases are still largely unknown, but mice models have been useful to study these mechanisms and explore the detailed effects of potential compounds. Such compounds usually have antioxidant and anti-inflammatory properties.
These properties are found in anthocyanins and a major source of dietary anthocyanins in Nordic diet is bilberries (European wild blueberries, Vaccinium myrtillus). In this explorative study we show results with differentially expressed genes in retina using a high-fat diet (HFD) mouse model. Our findings displayed differential regulation of genes in pathways for apoptosis, inflammation and oxidative stress, especially systemic lupus erythematosus (SLE), mitogen activated protein kinase (MAPK) and glutathione metabolism. Mice fed with HFD had increased retinal gene expression of several crystallins, which was reduced in the retina of mice fed with bilberries. Bilberries seem to reduce the expression of genes in MAPK and to increase the expression of genes in glutathione metabolism pathway. All together despite minor effects in the mouse phenotype, a diet rich in bilberries may prevent the retinal gene expression changes in the early stages of obesity.
Overall design Mice were fed ad libitum with normal control diet (NCD, 10% kcal fat), high-fat diet (HFD, 45% kcal fat), 5% (w/w) freeze-dried biberries (Vaccinium myrtillus) in NCD (NCD+BB) or HFD (HFD+BB) for 12 weeks. Diets were prepared in Research Diets Inc. Feed consumption and weight gain were measured during the feeding trial, and blood pressure and serum markers of obesity at the end.
Retinas were collected and RNA extracted from all 24 mice samples, and pooled retinas from 4 mice per group were hybridized with standard Illumina protocols. The expression in retinas was analyzed using R, Pathvisio and DAVID to screen for differences between high-fat and berry induced changes to control diets and further bilberry induced changes to HFD up- or downregulated transcripts.
Contributor(s) Mykkänen OT, Adriaens M, Kalesnykas G, Evelo CT, Törrönen R, Kaarniranta K
Citation(s) 22993483
Submission date Dec 05, 2011
Last update date Jun 14, 2018
Contact name Otto Taavetti Mykkänen
Organization name University of Eastern Finland
Department Public Health and Clinical Nutrition
Lab Food and Health Research Centre
Street address Yliopistonranta 1
City Kuopio
ZIP/Postal code 70211 Kuopio
Country Finland
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (4)
GSM843191 Retina of normal control diet (NCD)
GSM843192 Retina of high-fat diet (HFD)
GSM843193 Retina of 5% bilberries in normal control diet (NCD+BB)
BioProject PRJNA150049

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34154_RAW.tar 3.1 Mb (http)(custom) TAR
GSE34154_non-normalized.txt.gz 720.3 Kb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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