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| Status |
Public on Dec 31, 2014 |
| Title |
Liver gene expression signatures for type 2 diabetic mice and normal mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Over the past decade, considerable progress has been made regarding the discovery of gene targets by nanobiotechnologies. However, a huge gap frequently exists between candidate and therapeutic relevant genes due to the limitations associated with functional analyses. We report herein on the use of an integrated technologies involving a DNA microarray and an in vivo siRNA delivery system to rapidly and efficiently discover gene targets. Using DNA microarrays, we were able to show that the expression of a monoacylglycerol O-acyltransferase 1 (MOGAT1), an enzyme involved in triglyceride synthesis and storage, is elevated in the livers of obese diabetic mice. The in vivo silencing of hepatic Mogat1 via a non-viral siRNA delivery system resulted in a dramatic improvement in systemic glucose and lipid homeostasis. These interdisciplinary approaches have the potential to accelerate not only for identification of target gene, but also drug discovery and development.
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| Overall design |
Gene expression in liver was measured in 4- and 11-week-old KKAy and C57BL/6J mice.
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| Contributor(s) |
Hayashi Y, Kajimoto K, Kaji N, Baba Y, Harashima H |
| Citation(s) |
24643205 |
| Submission date |
Mar 10, 2012 |
| Last update date |
Sep 05, 2019 |
| Contact name |
Hayashi Yasuhiro |
| Organization name |
Hokkaido University
|
| Department |
Faculty of Pharmaceutical Sciences
|
| Street address |
Kita-12, Nishi-6, Kita-ku
|
| City |
Sapporo |
| ZIP/Postal code |
060-0812 |
| Country |
Japan |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA153213 |
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