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| Status |
Public on Mar 23, 2012 |
| Title |
Expression data from hepatocyte specific Klf6 knockout mice vs. control on high fat diet |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Dysregulated glucose homeostasis and lipid accumulation characterize non-alcoholic fatty liver disease (NAFLD), but underlying mechanisms are obscure. We report here that Krüppel-like factor 6 (KLF6), a ubiquitous transcription factor that promotes adipocyte differentiation, also provokes the metabolic abnormalities of NAFLD.
Mice with either hepatocyte-specific knockdown of KLF6 (DeltaHepKlf6) or global KLF6 heterozygosity (Klf6 +/-) have reduced body fat content and improved glucose and insulin tolerance. Mice with KLF6 depletion, compared to wild type mice, are protected from high fat diet-induced steatosis.
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| Overall design |
Three mice with a hepatocyte-specific knockdown of KLF6 (DeltaHepKlf6) on high fat diet and 3 littermate controls on the same diet were sacrificed after 8 weeks of diet. Liver tissue was preserved in RNAlater® (Ambion, Austin, TX). RNA was isolated from liver tissue and homogenized in TRIzol® reagent (Invitrogen, Carlsbad, CA). In order to identify potential KLF6 targets that contributed to changes in glucose- and lipid-metabolism, we performed an Affymetrix Exon1 S.T. Genearray® (Affymetrix, Santa Clara, CA).
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| Contributor(s) |
Bechmann LP, Vetter D, Ishida J, Hannivoort RA, Lee UE, Kocabayoglu P, Fiel MI, Muñoz U, Patman GL, Ge F, Yakar S, Li X, Agius L, Lee Y, Zhang W, Schwartz GJ, LeRoith D, Berk PD, Nagai R, Suzuki T, Reeves HL, Friedman SL |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 22, 2012 |
| Last update date |
Mar 06, 2018 |
| Contact name |
Weijia Zhang |
| E-mail(s) |
weijia.zhang@mssm.edu
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| Phone |
212-241-2883
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| Organization name |
Mount Sinai School of Medicine
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| Department |
Department of Medicine
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| Lab |
Bioinformatics Lab
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| Street address |
1425 Madison Avenue
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
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| Platforms (1) |
| GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA153799 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE36724_RAW.tar |
132.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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