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| Status |
Public on Oct 02, 2012 |
| Title |
Expression profile of PfmR (TTHB023)-deficient Thermus thermophilus HB8 strain |
| Organism |
Thermus thermophilus HB8 |
| Experiment type |
Expression profiling by array
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| Summary |
In order to find function of PfmR protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the PfmR-deficient strain grown in a rich medium at 70°C for 360 min.
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| Overall design |
Three PfmR-deficient T. thermophilus HB8 strains were each pre-cultured at 70°C for 16 h in 4 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (1.6 ml) were inoculated into 1 liter of the same medium and then cultivated at 70°C. Cells were collected after 360 min, and then, the expression of each mRNA was analyzed.
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| Contributor(s) |
Agari Y, Sakamoto K, Kuramitsu S, Shinkai A |
| Citation(s) |
22753056 |
| Submission date |
Mar 28, 2012 |
| Last update date |
Dec 19, 2012 |
| Contact name |
Akeo Shinkai |
| E-mail(s) |
ashinkai@spring8.or.jp, y_agari@spring8.or.jp
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| URL |
http://www.srg.harima.riken.jp/
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| Organization name |
RIKEN Harima Institute
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| Department |
SPring-8 Center
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| Street address |
1-1-1, Kouto, Sayo
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| City |
Hyogo |
| ZIP/Postal code |
679-5148 |
| Country |
Japan |
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| Platforms (1) |
| GPL9209 |
[TTHB8401a520105F] Riken Thermus thermophilus HB8 4K mRNA custom array, library Rev.2 |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE36912 |
SuperSeries for the study of expression analysis of the PfmR (TTHB023) in T. thermophilus HB8 |
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| Relations |
| BioProject |
PRJNA157005 |
