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| Status |
Public on Aug 02, 2012 |
| Title |
Cell type-specific chromatin profiling of Drosophila neurons [ChIP-Seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Chromatin profiling of nuclei isolated from genetically defined neuronal subpopulations of the adult Drosophila brain.
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| Overall design |
Cell type-specific histone modification maps were generated from nuclei isolated from all neurons (R57C10-GAL4), Kenyon cells (OK107-GAL4), and octopaminergic (Tdc2-GAL4) neurons using a method similar to INTACT (Deal and Henikoff, 2010; Steinner et al., 2012). Three histone modifications were profiled: H3K4me3, H3K27ac, and H3K27me3. Sequencing was performed with an Illumina HiSeq 2000.
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| |
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| Contributor(s) |
Henry GL, Davis FP, Picard S, Eddy SR |
| Citation(s) |
22855560 |
| Submission date |
Apr 04, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Fred P Davis |
| E-mail(s) |
davisf@janelia.hhmi.org
|
| Phone |
571-209-4000-3437
|
| Organization name |
HHMI Janelia Farm Research Campus
|
| Street address |
19700 Helix Dr
|
| City |
Ashburn |
| State/province |
VA |
| ZIP/Postal code |
20147 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE37033 |
Cell type-specific gene expression and chromatin profiling of Drosophila neurons |
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| Relations |
| BioProject |
PRJNA157885 |
| SRA |
SRP012052 |
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