|
| Status |
Public on Jan 25, 2013 |
| Title |
Expression data from 0.15M and 0.7M-fed wild-type and ChREBP mutant, third instar Drosophila larval fat bodies (FBs) |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Chronic high sugar feeding induces obesity, hyperglycemia, and insulin resistance in flies and mammals. These phenotypes are controlled by the fat body, a liver- and adipose- like tissue in Drosophila flies. To gain insight into the mechanisms underlying the connection between diet and insulin sensitivity, we used Illumina RNA-seq to profile gene expression in fat bodies isolated from chronically high sugar fed, wandering (post-prandial) third instar wild type larvae w(L3). These data were compared to control-fed wild-type wL3 fat bodies as well as those expressing transgenic interfering RNA (i) targeting CG18362 (Mio/dChREBP) in the fat body on both diets.
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| |
|
| Overall design |
Female VDRC w1118, cgGAL4, UAS-Dcr2 or UAS-ChREBPi(52606), cgGAL4, UAS-Dcr2 wandering third instar larvae were fed control (0.15M) or high (0.7M) sucrose and fat bodies isolated for RNA extraction.
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| |
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| Contributor(s) |
Baranski TJ, Brent MR, Musselman LP |
| Citation(s) |
23355467, 29487137 |
| Submission date |
Jan 24, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Thomas John Baranski |
| E-mail(s) |
tbaransk@dom.wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Street address |
660 S. Euclid Ave. Box 8127
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL11203 |
Illumina Genome Analyzer IIx (Drosophila melanogaster) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
|
| Samples (17)
|
| GSM1070027 |
wild-type wandering L3 FB_control diet-reared_biological rep 1 |
| GSM1070028 |
wild-type wandering L3 FB_control diet-reared_biological rep 2 |
| GSM1070029 |
wild-type wandering L3 FB_control diet-reared_biological rep 3 |
| GSM1070030 |
wild-type wandering L3 FB_control diet-reared_biological rep 4 |
| GSM1070031 |
wild-type wandering L3 FB_control diet-reared_biological rep 5 |
| GSM1070032 |
wild-type wandering L3 FB_control diet-reared_biological rep 6 |
| GSM1070033 |
ChREBP fat body knockdown, wandering L3 FB_control diet-reared_biological rep 1 |
| GSM1070034 |
ChREBP fat body knockdown, wandering L3 FB_control diet-reared_biological rep 2 |
| GSM1070035 |
ChREBP fat body knockdown, wandering L3 FB_control diet-reared_biological rep 3 |
| GSM1070036 |
wild-type wandering L3 FB_high-sugar diet-reared_biological rep 1 |
| GSM1070037 |
wild-type wandering L3 FB_high-sugar diet-reared_biological rep 2 |
| GSM1070038 |
wild-type wandering L3 FB_high-sugar diet-reared_biological rep 3 |
| GSM1070039 |
wild-type wandering L3 FB_high-sugar diet-reared_biological rep 4 |
| GSM1070040 |
wild-type wandering L3 FB_high-sugar diet-reared_biological rep 5 |
| GSM1070041 |
wild-type wandering L3 FB_high-sugar diet-reared_biological rep 6 |
| GSM1070042 |
ChREBP fat body knockdown wandering L3 FB_high-sugar diet-reared_biological rep 1 |
| GSM1070043 |
ChREBP fat body knockdown wandering L3 FB_high-sugar diet-reared_biological rep 2 |
|
| Relations |
| BioProject |
PRJNA187189 |
| SRA |
SRP018130 |
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