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| Status |
Public on Dec 02, 2013 |
| Title |
DamID of Dam::LMN-1 and Dam::EMR-1 in Caenorhabditis elegans adult worms |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
Laminopathies are caused by mutations in components of the nuclear envelope (NE). While most NE components are widely expressed, laminopathies affect only a subset of tissues. However, the understanding of the molecular mechanisms that explain this phenomenon is still elusive.
Here we have performed a genome wide DamID analysis in adult C. elegans nematodes comparing the DNA association profile of two components of the NE, Lamin/LMN-1 and Emerin/EMR-1. Although both proteins were associated to silent DNA, EMR-1 showed a predominant role in the anchoring of muscle and neuronal promoters to the nuclear periphery. Deletion of either EMR-1 or LEM-2, another integral NE protein, caused local changes in nuclear architecture with both increased and decreased LMN-1 association.
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| Overall design |
Comparison of Dam::LMN-1 and Dam::EMR-1 DNA assotiation in wild type strains and Dam::LMN-1 DNA association in wild type, lem-2(tm1582) and emr-1(gk119) mutant backgrounds.
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| Contributor(s) |
González-Aguilera C, Askjaer P |
| Citation(s) |
24490688 |
| Submission date |
Feb 08, 2013 |
| Last update date |
Mar 03, 2014 |
| Contact name |
Peter Askjaer |
| E-mail(s) |
pask@upo.es
|
| Phone |
+34 954 348 396
|
| Organization name |
Centro Andaluz de Biología del Desarrollo
|
| Street address |
UPO Carretera de Utrera km 1
|
| City |
Seville |
| ZIP/Postal code |
41013 |
| Country |
Spain |
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| Platforms (1) |
| GPL16504 |
NimbleGen C. elegans ChIP-chip 2.1M Whole-Genome Tiling Array [100718_Celegans180_ChIP_HX1] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA189179 |
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