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| Status |
Public on Mar 25, 2014 |
| Title |
Osteoblast Gene Expression in Response to Bicc1 Knockdown |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We recently identified Bicc1 as a regulator of osteoblast differentiation and bone mass. Bicc1 encodes an RNA-binding protein. Here, we have used siRNA to decrease Bicc1 expression in primary calvarial osteoblasts. Illlumina microarrays were then used to profile global gene expression changes.
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| Overall design |
The experiment consisted of 16 total samples in 4 treatment groups (N=4 replicates per treatment). The treatment groups were osteoblasts treated with 1). control scrambled siRNA, 2) Bicc1_1 siRNA, 3) Bicc1_2 siRNA and 3) Bicc1_3 siRNA. Cells were isolated, transfected and 48 hours later RNA was isolated and hybridized to arrays.
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| Contributor(s) |
Farber CR |
| Citation(s) |
24789909 |
| Submission date |
Oct 25, 2013 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Charles R Farber |
| E-mail(s) |
crf2s@virginia.edu
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| Organization name |
University of Virginia
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| Department |
Center for Public Health Genomics
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| Street address |
P.O. Box 800717
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| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (16)
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| GSM1250607 |
Primary calvarial osteoblast,Transfected with BICC1_2,RNA extracted 48 hours post-transfection,rep4 |
| GSM1250608 |
Primary calvarial osteoblast,Transfected with BICC1_1,RNA extracted 48 hours post-transfection,rep4 |
| GSM1250609 |
Primary calvarial osteoblast,Transfected with BICC1_1,RNA extracted 48 hours post-transfection,rep3 |
| GSM1250610 |
Primary calvarial osteoblast,Transfected with BICC1_2,RNA extracted 48 hours post-transfection,rep3 |
| GSM1250611 |
Primary calvarial osteoblast,Transfected with SC,RNA extracted 48 hours post-transfection,rep4 |
| GSM1250612 |
Primary calvarial osteoblast,Transfected with BICC1_3,RNA extracted 48 hours post-transfection,rep4 |
| GSM1250613 |
Primary calvarial osteoblast,Transfected with BICC1_3,RNA extracted 48 hours post-transfection,rep3 |
| GSM1250614 |
Primary calvarial osteoblast,Transfected with SC,RNA extracted 48 hours post-transfection,rep3 |
| GSM1250615 |
Primary calvarial osteoblast,Transfected with SC,RNA extracted 48 hours post-transfection,rep2 |
| GSM1250616 |
Primary calvarial osteoblast,Transfected with BICC1_2,RNA extracted 48 hours post-transfection,rep2 |
| GSM1250617 |
Primary calvarial osteoblast,Transfected with BICC1_1,RNA extracted 48 hours post-transfection,rep2 |
| GSM1250618 |
Primary calvarial osteoblast,Transfected with BICC1_3,RNA extracted 48 hours post-transfection,rep2 |
| GSM1250619 |
Primary calvarial osteoblast,Transfected with BICC1_1,RNA extracted 48 hours post-transfection,rep1 |
| GSM1250620 |
Primary calvarial osteoblast,Transfected with BICC1_2,RNA extracted 48 hours post-transfection,rep1 |
| GSM1250621 |
Primary calvarial osteoblast,Transfected with SC,RNA extracted 48 hours post-transfection,rep1 |
| GSM1250622 |
Primary calvarial osteoblast,Transfected with BICC1_3,RNA extracted 48 hours post-transfection,rep1 |
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| Relations |
| BioProject |
PRJNA224575 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE51693_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR |
| GSE51693_non-normalized.txt.gz |
1.6 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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