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Series GSE51693 Query DataSets for GSE51693
Status Public on Mar 25, 2014
Title Osteoblast Gene Expression in Response to Bicc1 Knockdown
Organism Mus musculus
Experiment type Expression profiling by array
Summary We recently identified Bicc1 as a regulator of osteoblast differentiation and bone mass. Bicc1 encodes an RNA-binding protein. Here, we have used siRNA to decrease Bicc1 expression in primary calvarial osteoblasts. Illlumina microarrays were then used to profile global gene expression changes.
 
Overall design The experiment consisted of 16 total samples in 4 treatment groups (N=4 replicates per treatment). The treatment groups were osteoblasts treated with 1). control scrambled siRNA, 2) Bicc1_1 siRNA, 3) Bicc1_2 siRNA and 3) Bicc1_3 siRNA. Cells were isolated, transfected and 48 hours later RNA was isolated and hybridized to arrays.
 
Contributor(s) Farber CR
Citation(s) 24789909
Submission date Oct 25, 2013
Last update date Jun 14, 2018
Contact name Charles R Farber
E-mail(s) crf2s@virginia.edu
Organization name University of Virginia
Department Center for Public Health Genomics
Street address P.O. Box 800717
City Charlottesville
State/province VA
ZIP/Postal code 22908
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM1250607 Primary calvarial osteoblast,Transfected with BICC1_2,RNA extracted 48 hours post-transfection,rep4
GSM1250608 Primary calvarial osteoblast,Transfected with BICC1_1,RNA extracted 48 hours post-transfection,rep4
GSM1250609 Primary calvarial osteoblast,Transfected with BICC1_1,RNA extracted 48 hours post-transfection,rep3
Relations
BioProject PRJNA224575

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51693_RAW.tar 3.1 Mb (http)(custom) TAR
GSE51693_non-normalized.txt.gz 1.6 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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