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Series GSE57477 Query DataSets for GSE57477
Status Public on Aug 15, 2014
Title Molecular subtyping of serous ovarian tumors reveals multiple connections to intrinsic breast cancer subtypes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Epithelial ovarian cancer is morphologically and clinically heterogeneous. Transcriptional profiling has revealed molecular subtypes (referred to as “C-signatures”) that correlate to biological as well as clinical features. We aimed to determine gene expression differences between malignant, benign and borderline serous ovarian tumors, and to investigate similarities to the intrinsic molecular subtypes of breast cancer. Global gene expression profiling was performed using Illumina's HT12 Bead Arrays and applied to 59 fresh-frozen ovarian tumors. SAM analysis revealed enrichment of cell cycel processes among the malignant tumors, in line with malignant tumors being highly proliferative. The borderline tumors were split between the malignant and benign tumor clusters, indicating that borderline tumors have both malignant and benign features. Furthermore, nearest centroid classification was performed applying previously published gene profiles for the ovarian cancer C-signatures and the intrinsic breast cancer subtypes, respectively, and showed significant correlations between the malignant serous tumors and the highly aggressive C1, C2 and C4 ovarian cancer signatures, and the basal-like breast cancer subtype. The benign and borderline serous tumors together were significantly correlated to the normal-like breast cancer subtype and the ovarian cancer C3 signature. The borderline tumors, on the other hand, correlated significantly to the Luminal A breast cancer subtype. These findings remained when analyzed in a large, independent dataset. The data in this study link the transcriptional profiles of serous ovarian cancer to the intrinsic molecular subtypes of breast cancer, in line with the shared clinical and molecular features between high-grade serous ovarian cancer and basal-like breast cancer, including an aggressive phenotype, frequent TP53 mutations and a high degree of genomic instability, and suggest that biomarkers and targeted therapies may overlap between these subsets of ovarian and breast cancers. Finally, the link between benign and borderline ovarian cancer and luminal breast cancer may indicate endocrine responsiveness in a subset of ovarian cancers.
 
Overall design Total RNA obtained from serous ovarian adenocarcinomas, adenomas and borderline tumors. Gene expression profiling using Illumina's HT12 v4 bead arrays. Application of ovarian cancer molecular subtypes and intrinsic breast cancer subtypes using nearest centroid classification. KRAS and BRAF mutation analyses in the malignant and borderline tumors.
 
Contributor(s) Jönsson J, Johansson I, Dominguez-Valentin M, Kimbung S, Jönsson M, Bonde JH, Måsbäck A, Malander S, Nilbert M, Hedenfalk I
Citation(s) 25226589
Submission date May 08, 2014
Last update date Aug 13, 2018
Contact name Jenny-Maria Jönsson
Organization name Lund University
Department Division of Oncology, Department of Clinical Sciences
Street address Scheelevägen 2
City Lund
ZIP/Postal code SE-22381
Country Sweden
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (72)
GSM1383467 Serous ovarian adenocarcinoma 106_pool.a1.lbe1
GSM1383468 Serous ovarian adenocarcinoma 118.a1.lbe1
GSM1383469 Serous ovarian adenocarcinoma 125.a1.lbe1
Relations
BioProject PRJNA246530

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57477_RAW.tar 26.2 Mb (http)(custom) TAR
GSE57477_non-normalized.txt.gz 19.8 Mb (ftp)(http) TXT
Processed data included within Sample table
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