 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 26, 2015 |
| Title |
Thrombospondin 1 in a model of colorectal carcinogenesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
By using a model of inflammation-induced carcinogenesis the effects of TSP-1 in induced tumors were analyzed. Mice received a single injection of azoxymethane (AOM) and multiple cycles of dextran sodium sulfate (DSS) for inducing chronic inflammation-related cancers. Proliferation and angiogenesis status were analyzed as well as their transcript profile by using a gene microarray approach.
We used Affymetrix GeneChips to determine the changes in the genetic profile between WT and TSP-1 deficient tumors in a model of colorectal carcinogenesis. We identified differentially expressed genes between WT and TSP-1 deficient tumors.
|
| |
|
| Overall design |
WT and TSP-1 deficient mice were with a single injection of AOM and were drinking 1.5%DSS (dextran sulfate sodium) to induce colitis in four cycles. Wt mice drinking water only was used as reference. Mice were sacrificed and tumors excised for these studies and morphological analyses
|
| |
|
| Contributor(s) |
Gutierrez L, Lopez-Dee Z, Chittur SV |
| Citation(s) |
26461935 |
| Submission date |
Aug 27, 2014 |
| Last update date |
Oct 09, 2019 |
| Contact name |
Sridar V Chittur |
| E-mail(s) |
schittur@albany.edu
|
| Phone |
518-591-7215
|
| Organization name |
SUNY-University at Albany
|
| Department |
Center for Functional Genomics
|
| Lab |
Microarray Core
|
| Street address |
One Discovery Drive, CRC 342G
|
| City |
Rensselaer |
| State/province |
NY |
| ZIP/Postal code |
12144 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (12)
|
| GSM812422 |
TSP-null-water, biological rep1 |
| GSM812423 |
TSP-null-water, biological rep2 |
| GSM812424 |
TSP-null-water, biological rep3 |
| GSM1489115 |
Wt,AOM-DSS treated, biological rep1 |
| GSM1489116 |
Wt,AOM-DSS treated, biological rep2 |
| GSM1489117 |
Wt,AOM-DSS treated, biological rep3 |
| GSM1489118 |
TSP-null, AOM-DSS treated, biological rep1 |
| GSM1489119 |
TSP-null, AOM-DSS treated, biological rep2 |
| GSM1489120 |
TSP-null, AOM-DSS treated, biological rep3 |
|
| Relations |
| BioProject |
PRJNA259597 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE60805_RAW.tar |
50.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...