|
| Status |
Public on Nov 04, 2015 |
| Title |
High Mobility Group protein mediated transcription requires DNA damage marker γ-H2AX |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The eukaryotic genome is organized into chromatin, which constitutes the physiological template for DNA-dependent processes including replication, recombination, repair and transcription. Chromatin mediated transcription regulation involves histone modifications, chromatin remodeling and DNA methylation. However, the precise biological function of non-histone chromatin-associated proteins is still unclear. The high mobility group proteins are the most abundant non-histone chromatin-associated proteins. Here we combined proteomic, ChIP-seq and transcriptome data to decipher the mechanism of transcriptional regulation mediated by the high mobility group AT-hook protein 2 (HMGA2). We showed that HMGA2-induced transcription requires H2AX phosphorylation at S139 (H2AXS139ph; γ-H2AX), mediated by the kinase ataxia telangiectasia mutated (ATM). Furthermore, we demonstrated the relevance of this mechanism within the biological context of TGFB1-signaling. Our results link H2AXS139ph, a marker for DNA damage, to transcription, which is a new function for this histone modification. The interplay between HMGA2, ATM and H2AX is a novel mechanism of transcription initiation.
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| |
|
| Overall design |
Chip-seq data of HMGA2, H2AXS139ph and ATM obtained from Mouse embryonic Fibroblast cells in wt and Ko of Hmga2
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| |
|
| Contributor(s) |
Barreto G, Singh I, Cordero J, Ozturk N |
| Citation(s) |
26045162 |
| Submission date |
Dec 04, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Guillermo Barreto |
| E-mail(s) |
guillermo.barreto@univ-lorraine.fr
|
| Organization name |
CNRS Director of Research
|
| Department |
Campus Biologie-Santé. Faculté de Médecine
|
| Lab |
Laboratoire IMoPA. UMR 7365 CNRS
|
| Street address |
9, Avenue de la Forêt de Haye ‐ BP 20199
|
| City |
Nancy Cedex |
| ZIP/Postal code |
54505 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA269287 |
| SRA |
SRP050556 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE63861_01_contigs_ATM_in_Hmga2_MEF_wt_vs_ATM_in_Hmga2_MEF_ko_in_promoter_wt.txt.gz |
357 b |
(ftp)(http) |
TXT |
| GSE63861_01_contigs_H2AXS139ph_in_Hmga2_MEF_wt_vs_H2AXS139ph_in_Hmga2_MEF_Ko.txt.gz |
373 b |
(ftp)(http) |
TXT |
| GSE63861_ATM_in_Hmga2_MEF_wt_peaks.bed.gz |
1.5 Mb |
(ftp)(http) |
BED |
| GSE63861_ATM_promoter_in_Hmga2_MEF_wt.bed.gz |
7.8 Kb |
(ftp)(http) |
BED |
| GSE63861_H2AXS139ph_and_ATM_in_Hmga2_MEF_wt_compared_HMGA2_in_MEF_Hmga2_wt_peaks.txt.gz |
170.8 Kb |
(ftp)(http) |
TXT |
| GSE63861_H2AXS139ph_in_Hmga2_MEF_wt_peaks.bed.gz |
1.5 Mb |
(ftp)(http) |
BED |
| GSE63861_H2AXS139ph_promoter_in_Hmga2_MEF_wt.bed.gz |
19.3 Kb |
(ftp)(http) |
BED |
| GSE63861_HMGA2_in_MEF_Hmga2_wt_peaks.bed.gz |
1.9 Mb |
(ftp)(http) |
BED |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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