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| Status |
Public on Jan 23, 2019 |
| Title |
mRNA profiling of human Multipotent Adipose-Derived Stem (hMADS) cells transfected with miR-29a and subjected to adipocyte differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The human microRNA hsa-miR-29a is a member of the miR-29 family of microRNAs, which is expressed in the adipogenic lineage. To identify putative direct target mRNAs of the miR-29 family, and to get an idea about potential functions of the miR-29 family in adipocyte development, we transfected human Multipotent Adipose-Derived Stem (hMADS) cells with miR-29a mimics (leading to elevation of intracellular levels of mature miR-29a). Subsequently, gene expression profiling was performed for hMADS cells at day 0 (48 h after transfection) and at day 3, i.e. 3 days after adipocyte differentiation was induced by a chemically defined medium.
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| Overall design |
Two-condition experiment, hMADS cells transfected either with miR-29a mimics or a control (non-targeting) miRNA mimic (miR-C). Separate analysis of two distinct time points after transfection: day 0 (i.e. 48 h after transfection; 4 biological replicates); day 3 after induction of adipocyte differentiation (3 biological replicates). Biological replicates were indepently grown, transfected and harvested. On each array, one biological replicate of miR-29a-transfected cells was directly compared to the corresponding miR-C-transfected cells (serving as reference sample). All hybridizations were repeated with reversed dye assignment (dye-swap) as technical replicates.
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| Contributor(s) |
Karbiener M, Scheideler M |
| Citation(s) |
30742779 |
| Submission date |
Feb 06, 2015 |
| Last update date |
Apr 24, 2019 |
| Contact name |
Michael Karbiener |
| E-mail(s) |
michael.karbiener@takeda.com
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| Organization name |
Takeda
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| Department |
Global Pathogen Safety
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| Street address |
Benatzkygasse 2-6 / G
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| City |
Vienna |
| ZIP/Postal code |
1221 |
| Country |
Austria |
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| Platforms (1) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA274752 |
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