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| Status |
Public on Sep 01, 2016 |
| Title |
JNK1 Ablation in Mice Confers Long-term Metabolic Protection from Diet-Induced Obesity at the Cost of Increased Skin Oxidative Damage |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Obesity and insulin resistance are associated with oxidative stress, which may be implicated in their progression. The kinase JNK1 emerged as a promising drug target for the treatment of obesity and type-2 diabetes. However, JNK1 is a key mediator of the oxidative stress response, promoting either cell dead or survival depending on magnitude and context of its activation. Furthermore, JNK inactivation shortens lifespan in drosophila and c. elegans. To learn on the safety and efficacy of long-term JNK inhibition in vertebrates, we investigated mice lacking JNK1 (JNK1-/-) exposed over a long period to an obesogenic high-fat diet (HFD).
JNK1-/- mice chronically fed an HFD developed more skin oxidative damage because of reduced catalase expression, but also showed sustained protection from obesity, adipose tissue inflammation, steatosis, and insulin resistance, paralleled by decreased oxidative damage in fat and liver. We conclude that JNK1 is a relatively safe drug target for obesity-related diseases.
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| Overall design |
RNA was collected from liver, skin and epididymal fat tissues from JNK1 KO mice and WT mice fed in high fat diet. Each condition was run in quadruplicate
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| Contributor(s) |
Becattini B, Zani F, Radici L, Breasson L, D'Agostino VG, Choo M, Provenzani A, Park JM, Solinas G, Fedrizzi T, Pancher M |
| Citation(s) |
27230858 |
| Submission date |
Oct 06, 2015 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Alessandro Provenzani |
| E-mail(s) |
alessandro.provenzani@unitn.it
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| Phone |
+390461283094
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| Organization name |
University of Trento
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| Department |
CIBIO
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| Lab |
Laboratory of Genomic Screening
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| Street address |
via delle regole 101
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| City |
Trento |
| ZIP/Postal code |
38100 |
| Country |
Italy |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA297836 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE73759_RAW.tar |
193.9 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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