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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 01, 2016 |
| Title |
Gene expression analysis of control and Tbx20 mutant Tie2Cre lineage from E12.5 hearts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate roles for Tbx20 in endocardium, we ablated Tbx20 utilizing Tie2Cre. Tie2Cre;Tbx20 mutants died at E14, exhibiting defects in multiple aspects of cardiac septation. Although endocardial cells lacking Tbx20 were able to undergo endothelial-to-mesenchymal transition, cushion mesenchymal cells lacking Tbx20 did not disperse normally. Non-cell autonomous roles of endocardial Tbx20 were also revealed, as evidenced by decreased myocardialization of outflow tract and failure of dorsal mesenchymal protrusion formation in mutants. To examine how ablation of Tbx20 in endocardial lineages affected gene expression, we performed global gene expression analysis on purified endocardial lineages. E12.5 hearts were dissociated, and Tie2Cre;RosatdTom lineage traced cells of controls and mutants were isolated by fluorescence activated cell sorting (FACS), after exclusion of blood cells (Ter119+, CD41+ and/or CD45+). Mutant endocardial lineages exhibited decreased expression of genes associated with extracellular matrix and cell migration.
E12.5 hearts were dissociated, and Tie2Cre;RosatdTom lineage traced cells of controls and mutants were isolated by fluorescence activated cell sorting (FACS), after exclusion of blood cells (Ter119+, CD41+ and/or CD45+).
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| Overall design |
FACS sorted Tie2Cre lineage from E12.5 hearts: Tie2Cre;Tbx20 +/loxP Control hearts versus Tie2Cre;Tbx20 loxP/- mutant hearts
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| Citation(s) |
27348591 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HL119967 |
Endocardial Pathways Regulated by Tbx20 |
UNIVERSITY OF CHICAGO |
NOBREGA |
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| Submission date |
Oct 08, 2015 |
| Last update date |
Jul 18, 2019 |
| Contact name |
Cornelis J. Boogerd |
| E-mail(s) |
k.boogerd@hubrecht.eu
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| Organization name |
Hubrecht Institute
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| Department |
Developmental Biology and Stem Cell Research
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| Street address |
Uppsalalaan 8
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| City |
Utrecht |
| ZIP/Postal code |
3584CT |
| Country |
Netherlands |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (2) |
| GSM1904547 |
Tie2Cre lineage E12.5 mouse hearts Tie2Cre;Tbx20 +/loxP |
| GSM1904548 |
Tie2Cre lineage E12.5 mouse hearts Tie2Cre;Tbx20 -/loxP |
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| Relations |
| BioProject |
PRJNA298232 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE73858_RAW.tar |
7.6 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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