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| Status |
Public on Nov 04, 2015 |
| Title |
Gene expression data from acetaminophen-induced toxicity in human hepatic in vitro systems and clinical liver samples |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In this study we conducted transcriptomics analyses of: (i) liver samples from patients suffering from acetaminophen-induced acute liver failure (n=3) and from healthy livers (n=2) and (ii) hepatic cell systems exposed to acetaminophen, including their respective vehicle controls. The investigated in vitro systems are: HepaRG cells, HepG2 cells and a novel human skinpostnatal stem cell-derived model i.e. human skin-precursors-derived hepatocyte-like cells (hSKP-HPC).
Clinical samples were obtained after surgical removal of the explant liver of patients diagnosed with ALF due to acetaminophen intoxication and treated by orthotopic liver transplantation (n=3). Samples from healthy livers were obtained from individuals deceased from brain damage (n=2).
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| Overall design |
Different samples of hSKP, HepG2 and HepaRG were obtained from the same cell batch of each cell system. All cells were exposed for 24 hours to their corresponding sub cytotoxic concentrations (IC10): IC10(hSKP-HPC)=18mM; IC10(HepaRG)=13mM; IC10(HepG2)=2mM. Experiments were conducted in triplicate.
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| Contributor(s) |
Rodrigues RM, Govaere O, Roskams T, Vanhaecke T, Rogiers V, De Kock J |
| Citation(s) |
27761495 |
| Submission date |
Oct 14, 2015 |
| Last update date |
Feb 04, 2021 |
| Contact name |
Robim M Rodrigues |
| E-mail(s) |
rmarceli@vub.ac.be
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| Organization name |
Vrije Universiteit Brussel
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| Department |
IVTD
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| Street address |
Laarbeeklaan 103
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| City |
Brussels |
| ZIP/Postal code |
1090 |
| Country |
Belgium |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (23)
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| GSM1907900 |
hSKP-HPC control, rep1 |
| GSM1907901 |
hSKP-HPC control, rep2 |
| GSM1907902 |
hSKP-HPC control, rep3 |
| GSM1907903 |
HepaRG exposed to APAP, rep1 |
| GSM1907904 |
HepaRG exposed to APAP, rep2 |
| GSM1907905 |
HepaRG exposed to APAP, rep3 |
| GSM1907906 |
HepaRG control, rep1 |
| GSM1907907 |
HepaRG control, rep2 |
| GSM1907908 |
HepaRG control, rep3 |
| GSM1907909 |
HepG2 exposed to APAP, rep1 |
| GSM1907910 |
HepG2 exposed to APAP, rep2 |
| GSM1907911 |
HepG2 exposed to APAP, rep3 |
| GSM1907912 |
HepG2 control, rep1 |
| GSM1907913 |
HepG2 control, rep2 |
| GSM1907914 |
HepG2 control, rep3 |
| GSM1907915 |
Liver sample APAP-induced ALF rep1 |
| GSM1907916 |
Liver sample APAP-induced ALF rep2 |
| GSM1907917 |
Liver sample APAP-induced ALF rep3 |
| GSM1907918 |
Liver sample control (healthy) rep1 |
| GSM1907919 |
Liver sample control (healthy) rep2 |
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| Relations |
| BioProject |
PRJNA298696 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE74000_RAW.tar |
100.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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