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| Status |
Public on Dec 01, 2021 |
| Title |
miRNA-9 regulates axon regeneration through the transcriptional regulator REST and the epigenetic factor UHRF1 [miRNA array] |
| Platform organism |
synthetic construct |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Injured sensory neurons successfully activate a pro-regenerative transcriptional program to enable axon regeneration and functional recovery, but the roles of genes that are inactivated after injury remain poorly understood. Analysis of the miRNA expression profile triggered by axon injury revealed down-regulation of the neural development associated miRNA, miR-9. A target of miR-9 is the critical epigenetic regulator involved in DNA methylation, ubiquitin-like containing PHD ring finger 1 (UHRF1), which increased after injury to promote axon regeneration. UHRF1 is known to repress the transcription of tumor suppressor genes through DNA methylation and we show that UHRF1 interacts with DNMT1 to methylate the promoter region of the tumor suppressors PTEN and CDKN1A, thereby triggering their inactivation. We also reveal that UHRF1 represses the transcriptional regulator REST. Our findings define an epigenetic mechanism that silences the transcription of axon growth suppressors to promote axon regeneration in sensory neurons.
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| Overall design |
miRNA expression profiling of spot cultures collected at Day in Vitro (DIV) 7, axotomized spot cultures at DIV7 collected at 1 hour, 3 hour, or 8 hours post injury.
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| Contributor(s) |
Oh YM, Mahar M, Larson D, Ewan EE, Cavalli V |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 23, 2016 |
| Last update date |
Dec 02, 2021 |
| Contact name |
Valeria Cavalli |
| E-mail(s) |
cavalli@pcg.wustl.edu
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| Organization name |
Washington University in St. Louis
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| Department |
Anatomy and Neurobiology
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| Street address |
660 S. Euclid Ave.
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| City |
Saint Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21572 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array [ProbeSet ID version] |
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| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE90477 |
Gene repression by DNA methylation promotes axon regeneration |
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| Relations |
| BioProject |
PRJNA354826 |
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