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| Status |
Public on Sep 21, 2007 |
| Title |
A screen to identify novel tumor suppressor genes silenced by methylation in melanoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Malignant melanoma is a common and frequently lethal disease. Current therapeutic interventions have little effect on survival, emphasizing the need for a better understanding of the genetic, epigenetic, and phenotypic changes in melanoma formation and progression. We identified genes that were not previously known to be silenced by methylation in melanoma using a microarray-based screen following treatment of melanoma cell lines with the DNA methylation inhibitor 5-Aza-2'-deoxycytidine.
Keywords: Expression changes following pharmacological reversal of epigenetic silencing
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| Overall design |
RNA was isolated following 0 and 48 hours of 5AzadC treatment of melanocytes and six melanoma cell lines (MelJuSo, UACC 903, C8161, Neo6 C8161, WM1205, and WM35) and used for the reexpression microarray analysis.
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| Contributor(s) |
Muthusamy V, Duraisamy S, Bradbury M, Hobbs C, Curley D, Nelson B, Bosenberg M |
| Citation(s) |
17145863 |
| Submission date |
Sep 20, 2007 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Viswanathan Muthusamy |
| E-mail(s) |
viswanathan.muthusamy@med.uvm.edu
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| Phone |
802 656 0914
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| Fax |
802 656 8892
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| Organization name |
University of Vermont
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| Department |
Pathology
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| Street address |
323A HSRF, 149 Beaumont Ave
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| City |
Burlington |
| State/province |
VT |
| ZIP/Postal code |
05405 |
| Country |
USA |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (14)
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| GSM230902 |
MelJuSo, 48h treatment with 5 AzadC |
| GSM230903 |
UACC903, no treatment |
| GSM230904 |
UACC903, 48h treatment with 5 AzadC |
| GSM230905 |
C8161, no treatment |
| GSM230906 |
C8161, 48h treatment with 5 AzadC |
| GSM230907 |
Neo6, no treatment |
| GSM230908 |
Neo6, 48h treatment with 5 AzadC |
| GSM230909 |
WM1205, no treatment |
| GSM230910 |
WM1205, 48h treatment with 5 AzadC |
| GSM230911 |
WM35, no treatment |
| GSM230912 |
WM35, 48h treatment with 5 AzadC |
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| Relations |
| BioProject |
PRJNA102651 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9118_RAW.tar |
132.3 Mb |
(http)(custom) |
TAR (of CEL, CHP, EXP) |
| Processed data included within Sample table |
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