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| Status |
Public on Mar 05, 2018 |
| Title |
BLIMP-1 is insufficient to induce antibody secretion in the absence of IRF4 |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by array
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| Summary |
Differentiation of B cells into antibody secreting cells (ASCs), plasmablasts and plasma cells, is regulated by a network of transcription factors. Within this network factors including PAX5 and BCL6 prevent ASC differentiation and maintain the B cell phenotype whereas BLIMP-1 and high IRF4 expression promote plasmacytic differentiation. BLIMP-1 is thought to induce immunoglobulin secretion. While IRF4 is needed for the survival of ASCs, its role in the regulation of antibody secretion has been controversial. BCL6-deficient DT40 B cell line has upregulated BLIMP-1 expression and secrete antibodies. In order to study the role of IRF4 in regulation of antibody secretion we have created a double knockout (DKO) DT40 B cell line deficient in both IRF4 and BCL6. This DKO cell line did not upregulate PRDM1 (the gene encoding for BLIMP-1) expression or secrete IgM. Even enforced BLIMP-1 expression in DKO cells or IRF4-deficient cells could not induce IgM secretion while it did in WT cells. However, enforced IRF4 expression in DKO cells induced strong IgM secretion. Our findings support a model where IRF4 expression in addition to BLIMP-1 expression is required to induce antibody secretion.
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| Overall design |
Gene expression profiles of BCL6 knockout (BCL6KO) and IRF4-BCL6 doubleknockout (DKO) chicken DT40 B cells were generated by Agilent Chicken Gene Expression Microarray V2.
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| Contributor(s) |
Budzynska PM, Kyläniemi MK |
| Citation(s) |
29430664 |
| Submission date |
Feb 13, 2017 |
| Last update date |
Jun 05, 2018 |
| Contact name |
Paulina Budzynska |
| Organization name |
University of Turku
|
| Street address |
Kiinamyllynkatu 13
|
| City |
Turku |
| ZIP/Postal code |
20520 |
| Country |
Finland |
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| Platforms (1) |
| GPL15357 |
Agilent-026441 Gallus gallus (Chicken) Oligo Microarray v2 (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA374575 |
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