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| Status |
Public on Nov 24, 2017 |
| Title |
Genome-wide profiling of somato-dendritic mRNA distribution in the hippocampus in RNG105/caprin1 conditional knockout (cKO) mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Background: mRNA localization and translation in neuronal dendrites are the basis for long-term synaptic plasticity and memory formation. RNA granules, membrane-less macromolecular assemblies in which dendritic mRNAs are recruited, are thought to contribute to these processes. RNG105/caprin1 is an RNA granule assembly factor involved in mRNA transport.
Results: Genome-wide profiling of mRNA distribution in distinct hippocampal layers revealed dendritically and somatically enriched mRNAs, and demonstrated marked reduction in the differential somato-dendritic localization of the mRNAs in RNG105 cKO mice.
Conclusion: RNG105 is an essential factor for the localization of many, but particularly for specific mRNAs localized to dendrites.
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| Overall design |
mRNAs isolated from stratum pyramidale (SP) and stratum radiatum (SR) in the CA1 region of the hippocampus from control mice and RNG105 cKO mice were deep sequenced in triplicate using a HiSeq1500 (Illumina).
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| Contributor(s) |
Ohashi R, Shigenobu S, Shiina N |
| Citation(s) |
29157358 |
| Submission date |
Mar 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Rie Ohashi |
| E-mail(s) |
ohashi@nibb.ac.jp
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| Organization name |
National Institute for Basic Biology
|
| Street address |
Nishigonaka 38, Myodaiji
|
| City |
Okazaki |
| ZIP/Postal code |
444-8585 |
| Country |
Japan |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA378997 |
| SRA |
SRP101802 |
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