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| Status |
Public on Apr 16, 2018 |
| Title |
De novo reconstruction of human adipose reveals conserved lncRNAs as regulators of brown adipogenesis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Obesity has emerged as a formidable health crisis due to its association with metabolic risk factors such as diabetes, dyslipidaemia and hypertension. Recent work has demonstrated the multifaceted roles of lncRNAs in regulating mouse adipose development, but its implication in human adipocytes remain largely unknown at least partially due to the lack of a comprehensive lncRNA catalog, particularly those specifically expressed in brown adipose tissue (BAT). In this study, we performed deep RNA-seq on adult subcutaneous, omental and fetal brown adipose tissues to de novo construct a catalog of 3,149 adipose active lncRNAs of which 1,351 are specifically detected in BAT. We further identified 318 lncRNAs conserved between human and mouse which, compared with non-conserved ones, are more broadly expressed in multiple cell types. One of these, lnc-dPRDM16, is transcribed divergently from Prdm16, tightly correlated with Prdm16 (R ≥ 0.7) in both mouse and human, and co-expressed (R ≥ 0.7) with protein-coding genes enriched in lipid and fatty acid catabolic processes. Loss of function of lnc-dPRDM16 led to a down-regulation of Prdm16 and an obvious reduction of adipogenesis in brown adipocyte culture. Together, our work has provided a comprehensive human adipose catalog built from diverse fat types, which when applied to our roadmap, identifies lnc-dPRDM16 as a promising modulator of adipose development for future clinical research.
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| Overall design |
Transcriptome profiling of BAT, OME and SUB samples
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| Contributor(s) |
Lim Y |
| Citation(s) |
29626186 |
| Submission date |
Mar 29, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Lei Sun |
| E-mail(s) |
sun.lei@duke-nus.edu.sg
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| Organization name |
Duke-NUS
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| Street address |
8 College Road
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| City |
Singapore |
| ZIP/Postal code |
169857 |
| Country |
Singapore |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA380957 |
| SRA |
SRP102710 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE97205_Human_brown_and_white_adipogenesis_FPKM.xlsx |
7.8 Mb |
(ftp)(http) |
XLSX |
| GSE97205_Human_fiBAT_OME_SUB_FPKM.xlsx |
8.1 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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