|
| Status |
Public on Sep 01, 2013 |
| Title |
CD4+ T cell of LP |
| Sample type |
RNA |
| |
|
| Source name |
lamina propria CD4+ T cells, IL-10 +/-
|
| Organism |
Mus musculus |
| Characteristics |
background/strain: C57BL/6
genotype: IL-10 +/-
gender: female
age: 10 weeks
tissue: lamina propria
cell type: CD4+ T cells
|
| Treatment protocol |
Single-cell suspensions of lamina propria lymphocytes were prepared by an enzymatic dissociation method using collagenase. CD4+ T cells of SP and LP were isolated by the MACS system (Miltenyi).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNA samples were extracted from cells using the miRNA Isolation kit (Ambion).
|
| Label |
Hy5
|
| Label protocol |
Extracted total RNA was labeled with Hy5 using the miRCURY LNA Array miR labeling kit (Exiqon, Vedbaek, Denmark).
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shaking (250 rpm). Hybridization buffer and washing protocol were according to the protocol supplied by TORAY Industries, Inc.
|
| Scan protocol |
ScanArray Express (PerkinElmer Japan Co., Ltd.) was used for scanning. Images were quantified using GenePix Pro version 6.0(Axon Instruments).
|
| Description |
IL-10 +/- mouse LPL.
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots' signal intensities of 95% confidence intervals. Measurements of both duplicate spots with the signal intensities greater than 2 standard deviations (SD) of the background signal intensity were considered to be valid. A relative expression level of a given miRNA was calculated by comparing the signal intensities of the averaged valid spots with their mean value throughout the microarray experiments.
|
| |
|
| Submission date |
Sep 14, 2012 |
| Last update date |
Sep 01, 2013 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13285 |
| Series (1) |
| GSE40891 |
miRNAs expressed in murine CD4+ T cells of splenocyte and lamina propria lymphocytes |
|
