|
| Status |
Public on Sep 29, 2012 |
| Title |
ID 25 DMSO |
| Sample type |
RNA |
| |
|
| Source name |
Preadipocytes from subcutaneous adipose tissue, fully differentiated and treated for 24 h with DMSO
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Subcutaneous adipose tissue
cell type: in vitro differentiated adipocytes
gender: Female
age: 52
bmi: 24.9
subject: Subject 2
|
| Treatment protocol |
Cells were treated for 24 h with 1 µM GW3965 or DMSO (vehicle)
|
| Growth protocol |
Preadipocytes were differentiated as described in PMID: 15925364 and 15492368
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted as in PMID: 19556420
|
| Label |
Biotin
|
| Label protocol |
RNA was prepared and hybridised biotinylated complementary RNA to Gene 1.0 ST Arrays using standardized protocols (Affymetrix Inc., Santa Clara, CA).
|
| |
|
| Hybridization protocol |
RNA was prepared and hybridised biotinylated complementary RNA to Gene 1.0 ST Arrays using standardized protocols (Affymetrix Inc., Santa Clara, CA).
|
| Scan protocol |
Scanning was performed using Affymetrix protocols (Affymetrix Inc., Santa Clara, CA).
|
| Description |
Subject 2
|
| Data processing |
Data was analysed using QCTool. All microarrays were subjected to an all-probe set scaling to target signal 100.
The GeneChip Operating Software Version 1.4 was used for analysis. Arrays were normalized using PLIER and all samples were subjected to an all-probeset scaling-to-target signal of 100.
|
| |
|
| Submission date |
Sep 28, 2012 |
| Last update date |
Sep 29, 2012 |
| Contact name |
Annie Pettersson |
| E-mail(s) |
annie.pettersson@ki.se
|
| Organization name |
Karolinska Institutet
|
| Department |
Dept. of medicine, Huddinge
|
| Street address |
Hälsovägen 7
|
| City |
Stockholm |
| ZIP/Postal code |
14186 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE41223 |
LXR activation induces insulin resistance in primary human adipocytes |
|
