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Status
Public on Mar 28, 2006
Title
HepG2-Neo-F4_Day 4
Sample type
RNA
Source name
pCMV transfected HepG2 cell, cultured for 4 days
Organism
Homo sapiens Characteristics
Plasmid pCMV transfected HepG2 cells, cultured for 4 days before array assay.
Growth protocol
Maintained in Dulbeccco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum, 250µg/ml G418, 100U/ml penicillin, 100µg/ml streptomycin, 2mM glutamine, 25mM HEPES solution, and 1mM sodium pyruvate at 37℃ under 5% CO2.
Extracted molecule
total RNA
Extraction protocol
Standard extraction protocol according to the TRIzol reagent manual.
Label
Biotin
Label protocol
Standard Affymetrix labelling protocol
Hybridization protocol
Standard Affymetrix hybridization protocol
Scan protocol
Standard Affymetrix scanning protocol
Description
Plasmid pCMV was transfected into the HepG2 cells. After screened by G418, stable cell clones were selected and used for array.
Data processing
Affymetrix "Statistical Algorithms Reference Guide" describes the details of data processing
Submission date
Mar 23, 2006
Last update date
Mar 27, 2006
Contact name
Xiaochen Tian
E-mail(s)
xiaochentian@hotmail.com Phone
86-21-54237572
URL
http://mvlab-fudan.cn/ Organization name
Fudan University
Department
Shanghai Medical college
Lab
lab of medical molecular virology
Street address
No.138 Yixueyuan Road
City
Shanghai
ZIP/Postal code
200032
Country
China
Platform ID
GPL570 Series (1)
GSE4549 Global exploration of interaction between HBsAg expression and Hepatocytes
Data table header descriptions ID_REF VALUE A measure of the relative abundance of a transcript.
ABS_CALL A measurement indicating if the transcript was detected (Present), not detected (Absent), or marginally detected (Marginal). Generally, p-value<0.04 is Present; 0.04<p-value<0.06 is Marginal; p-value>0.06 is Absent.
DETECTION P-VALUE A Detection p-value measures the probability that the discrimination scores of all probe pairs in the probe set are above a certain level, and that the target is likely to be Present.
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
140.1
P
0.000195
AFFX-BioB-M_at
188.2
P
0.000044
AFFX-BioB-3_at
86.2
P
0.000169
AFFX-BioC-5_at
258.6
P
0.000044
AFFX-BioC-3_at
280.8
P
0.000044
AFFX-BioDn-5_at
833.8
P
0.000044
AFFX-BioDn-3_at
1711.1
P
0.000044
AFFX-CreX-5_at
3515.6
P
0.000044
AFFX-CreX-3_at
4229.9
P
0.000044
AFFX-DapX-5_at
6.2
A
0.205732
AFFX-DapX-M_at
5.6
A
0.382599
AFFX-DapX-3_at
0.4
A
0.963431
AFFX-LysX-5_at
0.9
A
0.686277
AFFX-LysX-M_at
7.9
A
0.239044
AFFX-LysX-3_at
4.8
A
0.275146
AFFX-PheX-5_at
2.9
A
0.239063
AFFX-PheX-M_at
0.5
A
0.966323
AFFX-PheX-3_at
1.7
A
0.772364
AFFX-ThrX-5_at
3.3
A
0.659352
AFFX-ThrX-M_at
5
A
0.354441
54675 1392 Kbytes .Supplementary data files not provided
