|
| Status |
Public on Jan 01, 2014 |
| Title |
hESC H1 B2M KO c2 |
| Sample type |
RNA |
| |
|
| Source name |
Cultured hESC H1 B2M KO
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
cell type: hESC H1 cells
genotype/variation: B2M KO
|
| Treatment protocol |
NA
|
| Growth protocol |
All cells were cultured on Matrigel (BD Biosciences) in mTESR media (Stem Cell Technologies)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent. Quality control was performed with Agilent 2100 Bioanalyzers and NanoDrop DN-1000 spectrophotometer.
|
| Label |
biotin
|
| Label protocol |
All RNA samples were amplified and labelled using Illumina's TotalPrep RNA Amplification Kit according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
cRNA samples were hybridized onto Human HT-12 v4 Expression BeadChip (Illumina) according to manufacture's protocols.
|
| Scan protocol |
Arrays were scanned by the Genomics Resource Lab at Fred Hutchinson Cancer Center with an Illumina BeadArray Reader.
|
| Description |
hESC H1 cells were targeted with AAV-based vectors to knock out B2M gene, clone 2
Sample name: B2MKOc2
|
| Data processing |
Raw expression files were loaded into Bioconductor. The Bioconductor package 'lumi' was used to read in the GenomeStudio Probe Level from unnormalized data, and quantile normalize the data.
|
| |
|
| Submission date |
Nov 14, 2012 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Laura Riolobos |
| E-mail(s) |
lriolobo@uw.edu
|
| Organization name |
University of Washington
|
| Street address |
1959 NE Pacific St
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE42289 |
Gene Expression analysis of hESC B2M knock-out |
|
