|
| Status |
Public on Dec 05, 2012 |
| Title |
cellline1_rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
miR-29b transfected cell line 1
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
cell type: Fibroblast
|
| Treatment protocol |
Asynchronously proliferating fibroblasts were reverse transfected with either miR-29b or a microRNA negative control Pre-miR from Ambion.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated from fibroblasts 48 hours post-transfection using mirVana total RNA isolation kit and procedure.
|
| Label |
Cy5
|
| Label protocol |
Total RNA extracts were cRNA labeled using the Agilent Low RNA Input Fluorescent Labeling Kit according to the manufacturer's protocol
|
| |
|
| Channel 2 |
| Source name |
Negative control transfected cell line 1
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
cell type: Fibroblast
|
| Treatment protocol |
Asynchronously proliferating fibroblasts were reverse transfected with either miR-29b or a microRNA negative control Pre-miR from Ambion.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated from fibroblasts 48 hours post-transfection using mirVana total RNA isolation kit and procedure.
|
| Label |
Cy3
|
| Label protocol |
Total RNA extracts were cRNA labeled using the Agilent Low RNA Input Fluorescent Labeling Kit according to the manufacturer's protocol
|
| |
|
| |
| Hybridization protocol |
Labeled cRNA were hybridized to arrays according to Agilent's manufacturer's protocol
|
| Scan protocol |
Scanned using Agilent G2505B array scanner according to manufacturer's protocol.
|
| Data processing |
Probes were filtered to eliminate those with either a signal intensity below 350 or flagged by Feature Extractor for saturation or no signal above background in any hybridization. Replicated signals from identical sequence-probes were averaged. For each array, the ratio of Cy5 to Cy3 (red to green) was log2 transformed and zero-centered.
|
| |
|
| Submission date |
Nov 29, 2012 |
| Last update date |
Dec 05, 2012 |
| Contact name |
Eric Jungwoo Suh |
| Organization name |
Princeton University
|
| Department |
Molecular Biology
|
| Lab |
Coller Lab
|
| Street address |
14 Washington Rd
|
| City |
Princeton |
| State/province |
NJ |
| ZIP/Postal code |
08544 |
| Country |
USA |
| |
|
| Platform ID |
GPL6480 |
| Series (2) |
| GSE42613 |
A microRNA network regulates proliferative timing and extracellular matrix synthesis during cellular quiescence in fibroblasts [mRNA] |
| GSE42614 |
A microRNA network regulates proliferative timing and extracellular matrix synthesis during cellular quiescence in fibroblasts |
|
