|
| Status |
Public on Aug 30, 2013 |
| Title |
MTEC PR8 infected rep4 [C57Bl/6-Mx IFNAR1-/IL-28ra-/-] |
| Sample type |
RNA |
| |
|
| Source name |
tracheas from C57Bl/6-Mx IFNAR1-/IL-28ra-/-
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57Bl/6-Mx
tissue: trachea
genotype: IFNAR1-/-IL-28ra-/-
|
| Treatment protocol |
MTEC were either mock infected or infected with PR8 (moi=0.3)
|
| Growth protocol |
Primary cells isolated from tracheas by enzymatic treatment were seeded onto 0.4 μm pore size clear polyester membrane coated with a collagen solution. At confluence, media was removed from the upper chamber to establish an air- liquid interface (ALI). Fully differentiated, 10-14 days-old post ALI cultures were routinely used for experiments.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from MTEC cultures by directly lysing the cells in the transwells, using the Qiagen RNeasy mini kit
|
| Label |
biotin
|
| Label protocol |
User guide Affymetrix GeneChip 3’ Express Kit (P/N 702646 Rev.8)
|
| |
|
| Hybridization protocol |
User guide Affymetrix GeneChip 3’ Express Kit (P/N 702646 Rev.8)
|
| Scan protocol |
User guide Affymetrix Expression Wash, Stain and Scan (P/N 702731 Rev. 3)
|
| Description |
Gene expression data from MTEC cells
|
| Data processing |
Primary and QC analysis on Affymetrix Expression Console V1.1. Data were normalized to the median of mock infected sample (all genotypes)
|
| |
|
| Submission date |
Jan 24, 2013 |
| Last update date |
Aug 30, 2013 |
| Contact name |
Stefania Crotta |
| E-mail(s) |
stefania.crotta@crick.ac.uk
|
| Phone |
++44 208 8162260
|
| Organization name |
The Crick- Mill Hill Laboratories
|
| Department |
IR
|
| Street address |
The Ridgeway
|
| City |
London |
| ZIP/Postal code |
NW71AA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE43708 |
Expression data from Influenza A infected mouse primary tracheal epithelial cell cultures (MTEC), from wild-type, IFNAR1-/-, IL28Ra-/- and IFNAR1-/- IL28Ra-/- double ko |
| GSE43710 |
Type I and type III interferons drive redundant amplification loops to induce a transcriptional signature in influenza-infected airway epithelia |
|
